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Considerations When Using Array Technologies for Male Factor Assessment

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The Genetics of Male Infertility

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Abstract

Expression profiles from sets of genes are currently being explored as candidate diagnostics to assess male fertility status and as surrogate makers of paternal toxicological exposure. In this chapter, we describe considerations for their design when using microarrays to create a clinical diagnostic tool. Two commercially available oligonucleotide-based platforms were compared. The results are then referenced against an expressed sequence tag data set and a cDNA array. The concordance between the different platforms for genes indicated as present with high confidence and absent with high confidence when provided with the same pool of RNA is presented. Based on these data, the capacity for this technology to develop into a robust diagnostic for male factor fertility is discussed.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI

    Adrian E. Platts BSC

  2. National Center for Computational Toxicology, Office of Research and Development, U.S. Environmental Protection Agency, Durham, NC

    David J. Dix PhD

  3. Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Institute for Scientific Computing, Wayne State University School of Medicine, Detroit, MI

    Stephen A. Krawetz PhD

Authors
  1. Adrian E. Platts BSC
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  2. David J. Dix PhD
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  3. Stephen A. Krawetz PhD
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Editor information

Editors and Affiliations

  1. Departments of Surgery (Urology), Obstetrics and Gynecology, and Physiology, University of Utah School of Medicine, Salt Lake City, UT

    Douglas T. Carrell PhD

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© 2007 Humana Press Inc.

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Platts, A.E., Dix, D.J., Krawetz, S.A. (2007). Considerations When Using Array Technologies for Male Factor Assessment. In: Carrell, D.T. (eds) The Genetics of Male Infertility. Humana Press. https://doi.org/10.1007/978-1-59745-176-5_3

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