Abstract
Wide angle X-ray scattering is a key technique for the analysis of amyloid fibrils that can be used to confirm the presence of a characteristic cross-beta fibril structure and to characterise the arrangement of beta-strands and beta-sheets within this fibril core. Further structural insight can be obtained by the comparison of X-ray scattering data obtained for dried and hydrated fibril samples. We describe simple techniques for the preparation of dried and hydrated fibril samples for X-ray analysis and the subsequent analysis of X-ray scattering patterns using custom built and readily available software.
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Gras, S.L., Squires, A.M. (2011). Dried and Hydrated X-Ray Scattering Analysis of Amyloid Fibrils. In: Hill, A., Barnham, K., Bottomley, S., Cappai, R. (eds) Protein Folding, Misfolding, and Disease. Methods in Molecular Biology, vol 752. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-223-0_10
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DOI: https://doi.org/10.1007/978-1-60327-223-0_10
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Publisher Name: Humana Press, Totowa, NJ
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