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Sedimentation Velocity Analysis of Amyloid Fibrils

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Protein Folding, Misfolding, and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 752))

Abstract

Analytical ultracentrifugation is a classical technique used to study the solution behavior of proteins. Experimentally determined sedimentation coefficients provide information regarding the size, shape, and interactions of biological macromolecules. Sedimentation velocity methods have been used to characterize the different aggregation states of amyloid oligomers and fibrils. This chapter first describes the theoretical background for sedimentation velocity analysis. It then provides experimental protocols for sedimentation velocity experiments using the analytical ultracentrifuge. Finally, this chapter describes the procedure used to analyze sedimentation velocity data to obtain the size distribution of amyloid fibrils and their oligomeric precursors.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia

    Geoffrey J. Howlett

Authors
  1. Chi Le Lan Pham
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  2. Yee-Foong Mok
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  3. Geoffrey J. Howlett
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Corresponding author

Correspondence to Geoffrey J. Howlett .

Editor information

Editors and Affiliations

  1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Andrew F. Hill

  2. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Kevin J. Barnham

  3. , Department of Biochemistry &, Monash University, Wellington Road, Clayton, 3800, Victoria, Australia

    Stephen P. Bottomley

  4. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Roberto Cappai

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Pham, C.L.L., Mok, YF., Howlett, G.J. (2011). Sedimentation Velocity Analysis of Amyloid Fibrils. In: Hill, A., Barnham, K., Bottomley, S., Cappai, R. (eds) Protein Folding, Misfolding, and Disease. Methods in Molecular Biology, vol 752. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-223-0_12

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  • DOI: https://doi.org/10.1007/978-1-60327-223-0_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-221-6

  • Online ISBN: 978-1-60327-223-0

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