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Dendritic Cell Protocols pp 299–318Cite as

Mouse Models of Viral Infection: Influenza Infection in the Lung

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 595))

Abstract

Respiratory viral infections are a major cause of morbidity and mortality. Protection of the respiratory tract from pathogen infections, such as influenza virus, requires the orchestrated activation and trafficking of pulmonary dendritic cells (DCs) from the lung to the lymph node (LN) in order to ensure optimized T-cell responses. Gaining a better understanding of the cellular and molecular processes that protect the lung during infection is essential for future advances in vaccine strategies and treatments. Influenza viral infection in mice offers a very well-defined immunological system in which the underlying parameters regulating the generation of protective immunity can be elucidated. In this chapter, we review methods for quantitative analysis of DC and T-cell responses in a murine model infection of influenza. Antigen-specific tracking and quantitation of viral immune responses have been greatly facilitated by the advent of MHC tetramers and intracellular cytokine analysis, together with gentle isolation procedures for dendritic cells allowing detection of viral and endogenous antigens.

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Acknowledgements

Research in the author’s laboratory is supported by grants from the National Health and Medical Research Council (Australia), the Howard Hughes Medical Institute (USA), and the Sylvia and Charles Viertel Foundation (Australia).

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  1. The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia

    Adele M. Mount & Gabrielle T. Belz

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  1. Adele M. Mount
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  1. Quellijnstraat 25A, Amsterdam, 1072 XN, Netherlands

    Shalin H. Naik

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Mount, A.M., Belz, G.T. (2010). Mouse Models of Viral Infection: Influenza Infection in the Lung. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_20

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  • DOI: https://doi.org/10.1007/978-1-60761-421-0_20

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