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Solid-Phase Peptide Synthesis Using Microwave Irradiation

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Drug Design and Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 716))

Abstract

Since the advent of solid-phase peptide synthesis (SPPS) in the late 1950s, numerous advancements in the underlying chemistry (i.e., orthogonal protection strategy, coupling reagents, and solid support matrices) have greatly improved the efficiency of the technique. More recently, application of microwave radiation to SPPS has been found to reduce reaction time and/or increase the initial purity of synthetic peptide products. In this protocol, conditions are described to accomplish rapid peptide coupling and 9-fluorenylmethoxycarbonyl (Fmoc) removal reactions under temperature-controlled conditions in either a manual or automated synthesis format using a microwave reactor. These microwave-assisted peptide synthesis procedures have been used to rapidly prepare a “difficult” peptide sequence from the acyl carrier protein, ACP(65-74), in less than 3 h and the reduced, linear precursor to human hepcidin, in high initial purity.

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Authors and Affiliations

  1. Peptide Research & Discovery, Amgen, Thousand Oaks, CA, USA

    Justin K. Murray & Jennifer Aral

  2. Peptide Research and Discovery, Amgen, Thousand Oaks, CA, USA

    Les P. Miranda

Authors
  1. Justin K. Murray
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  2. Jennifer Aral
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  3. Les P. Miranda
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Corresponding author

Correspondence to Les P. Miranda .

Editor information

Editors and Affiliations

  1. College of Pharmacy, Dept. Basic Pharmaceutical Sciences, University of Louisiana at Monroe, Bienville Drive 1800, Monroe, 71201, Louisiana, USA

    Seetharama D. Satyanarayanajois

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Murray, J.K., Aral, J., Miranda, L.P. (2011). Solid-Phase Peptide Synthesis Using Microwave Irradiation. In: Satyanarayanajois, S. (eds) Drug Design and Discovery. Methods in Molecular Biology, vol 716. Humana Press. https://doi.org/10.1007/978-1-61779-012-6_5

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  • DOI: https://doi.org/10.1007/978-1-61779-012-6_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-011-9

  • Online ISBN: 978-1-61779-012-6

  • eBook Packages: Springer Protocols

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