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Neurodegeneration pp 99–110Cite as

Neural Differentiation of Induced Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 793))

Abstract

The great potential of induced pluripotent cells (iPS) cells is that it allows the possibility of deriving pluripotent stem cells from any human patient. Generation of patient-derived stem cells serves as a great source for developing cell replacement therapies and also for creating human cellular model systems of specific diseases or disorders. This is only of benefit if there are well-established differentiation assay systems to generate the cell types of interest. This chapter describes robust and well-characterized protocols for differentiating iPS cells to neural progenitors, neurons, glia and neural crest cells. These established assays can be applied to iPS cell lines derived from patients with neurodegenerative disorders to study cellular mechanisms associated with neurodegeneration as well as investigating the regenerative potential of patient derived stem cells.

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References

  1. Chiba, S., Lee, Y. M., Zhou, W., and Freed, C. R. (2008) Noggin enhances dopamine neuron production from human embryonic stem cells and improves behavioral outcome after transplantation into Parkinsonian rats. Stem Cells. 26, 2810–2820.

    Article  PubMed  CAS  Google Scholar 

  2. Pera, M. F., Andrade, J., Houssami, S., Reubinoff, B., Trounson, A., Stanley, E. G., Ward-van Oostwaard, D., and Mummery, C. (2004) Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin. J Cell Sci. 117, 1269–1280.

    Article  PubMed  CAS  Google Scholar 

  3. Pomp, O., Brokhman, I., Ben-Dor, I., Reubinoff, B., and Goldstein, R. S. (2005) Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells. Stem Cells. 23, 923–930.

    Article  PubMed  CAS  Google Scholar 

  4. Chambers, S. M., Fasano, C. A., Papapetrou, E. P., Tomishima, M., Sadelain, M., and Studer, L. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nature Biotechnol. 27, 275–280.

    Article  CAS  Google Scholar 

  5. Gerrard, L., Rodgers, L., and Cui, W. (2005) Differentiation of human embryonic stem cells to neural lineages in adherent culture by blocking bone morphogenetic protein signaling. Stem Cells. 23, 1234–1241.

    Article  PubMed  CAS  Google Scholar 

  6. Karumbayaram, S., Novitch, B. G., Patterson, M., Umbach, J. A., Richter, L., Lindgren, A., Conway, A. E., Clark, A. T., Goldman, S. A., Plath, K., Wiedau-Pazos, M., Kornblum, H. I., and Lowry, W. E. (2009) Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells. 27, 806–811.

    Article  PubMed  CAS  Google Scholar 

  7. Cooper, O., Hargus, G., Deleidi, M., Blak, A., Osborn, T., Marlow, E., Lee, K., Levy, A., Perez-Torres, E., Yow, A., and Isacson, O. Differentiation of human ES and Parkinson’s disease iPS cells into ventral midbrain dopaminergic neurons requires a high activity form of SHH, FGF8a and specific regionalization by retinoic acid. Mol Cell Neurosci. 45(3), 258–266.

    Google Scholar 

  8. Vazin, T., Chen, J., Lee, C. T., Amable, R., and Freed, W. J. (2008) Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells. Stem Cells. 26, 1517–1525.

    Article  PubMed  Google Scholar 

  9. Davidson, K. C., Jamshidi, P., Daly, R., Hearn, M. T., Pera, M. F., and Dottori, M. (2007) Wnt3a regulates survival, expansion, and maintenance of neural progenitors derived from human embryonic stem cells. Mol Cell Neurosci. 36, 408–415.

    Article  PubMed  CAS  Google Scholar 

  10. Pomp, O., Brokhman, I., Ziegler, L., Almog, M., Korngreen, A., Tavian, M., and Goldstein, R. S. (2008) PA6-induced human embryonic stem cell-derived neurospheres: a new source of human peripheral sensory neurons and neural crest cells. Brain Res. 1230, 50–60.

    Article  PubMed  CAS  Google Scholar 

  11. Denham, M., Thompson, L. H., Leung, J., Pebay, A., Bjorklund, A., and Dottori, M. (2010) Gli1 is an inducing factor in generating floor plate progenitor cells from human embryonic stem cells. Stem Cells. 28(10), 1805–1815.

    Google Scholar 

  12. Hotta, R., Pepdjonovic, L., Anderson, R. B., Zhang, D., Bergner, A. J., Leung, J., Pebay, A., Young, H. M., Newgreen, D. F., and Dottori, M. (2009) Small-molecule induction of neural crest-like cells derived from human neural progenitors. Stem Cells. 27, 2896–2905.

    PubMed  CAS  Google Scholar 

  13. Li, X. J., Du, Z. W., Zarnowska, E. D., Pankratz, M., Hansen, L. O., Pearce, R. A., and Zhang, S. C. (2005) Specification of motoneurons from human embryonic stem cells. Nat. Biotechnol. 23, 215–221.

    Article  PubMed  Google Scholar 

  14. Yan, Y., Yang, D., Zarnowska, E. D., Du, Z., Werbel, B., Valliere, C., Pearce, R. A., Thomson, J. A., and Zhang, S. C. (2005) Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells. Stem Cells. 23, 781–790.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Centre for Neuroscience, University of Melbourne, Parkville, VIC, Australia

    Mark Denham & Mirella Dottori

  2. Department of Pharmacology, University of Melbourne, Parkville, VIC, Australia

    Mirella Dottori

Authors
  1. Mark Denham
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  2. Mirella Dottori
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Corresponding author

Correspondence to Mirella Dottori .

Editor information

Editors and Affiliations

  1. Weill Medical College, Dept. Neurology & Neuroscience, Cornell University, East 68th St. 525, New York, 10065, New York, USA

    Giovanni Manfredi

  2. York Avenue 1300, New York, 10065, New York, USA

    Hibiki Kawamata

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© 2011 Springer Science+Business Media, LLC

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Denham, M., Dottori, M. (2011). Neural Differentiation of Induced Pluripotent Stem Cells. In: Manfredi, G., Kawamata, H. (eds) Neurodegeneration. Methods in Molecular Biology, vol 793. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-328-8_7

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  • DOI: https://doi.org/10.1007/978-1-61779-328-8_7

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-327-1

  • Online ISBN: 978-1-61779-328-8

  • eBook Packages: Springer Protocols

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