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Analysis of Assembly of Secreted Mucins

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Book cover Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 842))

Abstract

Studies of assembly and secretion of gel-forming mucins are complex. The pulse-chase methods for mucins described here include metabolic radiolabeling and labeling in animals with azido-GalNAc. The labeled mucins are analyzed by composite agarose-polyacrylamide gel electrophoresis and autoradiography or by mucus-preserving tissue fixation and Click-iT® chemistry.

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Acknowledgments

This work was supported by the Swedish Research Council (no. 7461, 21027, and 342-2004-4434), The Swedish Cancer Foundation, The Knut and Alice Wallenberg Foundation (KAW2007.0118), IngaBritt and Arne Lundberg Foundation, Sahlgren’s University Hospital (LUA-ALF), EU-FP7 IBDase (no. 200931), Wilhelm and Martina Lundgren’s Foundation, The Swedish CF Foundation, Torsten och Ragnar Söderbergs Stiftelser, and The Swedish Foundation for Strategic Research—The Mucosal Immunobiology and Vaccine Center (MIVAC) and the Mucus-Bacteria-Colitis Center (MBC) of the Innate Immunity Program (2010–2014).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Mucin Biology Group, University of Gothenburg, Gothenburg, Sweden

    Malin E. V. Johansson & Gunnar C. Hansson

Authors
  1. Malin E. V. Johansson
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  2. Gunnar C. Hansson
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Corresponding author

Correspondence to Gunnar C. Hansson .

Editor information

Editors and Affiliations

  1. Mucosal Diseases Program, Mater Medical Research Institute, Aubigny Place, South Brisbane, 4101, Queensland, Australia

    Michael A. McGuckin

  2. Fac. Life Sciences, Wellcome Trust Centre for, University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom

    David J. Thornton

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Johansson, M.E.V., Hansson, G.C. (2012). Analysis of Assembly of Secreted Mucins. In: McGuckin, M., Thornton, D. (eds) Mucins. Methods in Molecular Biology, vol 842. Humana Press. https://doi.org/10.1007/978-1-61779-513-8_6

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  • DOI: https://doi.org/10.1007/978-1-61779-513-8_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-512-1

  • Online ISBN: 978-1-61779-513-8

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