Abstract
Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
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Acknowledgments
Our special thanks for excellent technical support to Christin Gräfe, Livia Schulze, and Barbara Uteß (Institute Immunology, Dresden, Germany).
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© 2012 Springer Science+Business Media, LLC
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Michalk, I., Koristka, S., Arndt, C., Bachmann, M. (2012). Isolation of Proteins from Polyacrylamide Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_35
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DOI: https://doi.org/10.1007/978-1-61779-821-4_35
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-820-7
Online ISBN: 978-1-61779-821-4
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