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Detection and Characterisation of Alloreactive T Cells

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Immunogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 882))

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Abstract

T cell alloreactivity is responsible for much of the morbidity and mortality associated with tissue transplantation and graft versus host disease. Immunoassays for ex vivo monitoring and quantitation of alloreactive T cells are being increasingly utilised to provide valuable information for individualised clinical management of transplant recipients. Here we describe detailed methodologies for both traditional and novel assays utilised for the detection, quantitation, and functional characterisation of alloreactive T cells and highlight the key advantages and disadvantages of each system.

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Acknowledgements

The authors would like to thank Ms Patricia Therese Illing, The University of Melbourne, Australia, for her help in proofreading the manuscript.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC, Australia

    Mandvi Bharadwaj & James McCluskey

  2. Department of Medicine, Monash University, Alfred Hospital, Melbourne, VIC, Australia

    Nicole A. Mifsud

Authors
  1. Mandvi Bharadwaj
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  2. Nicole A. Mifsud
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  3. James McCluskey
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Correspondence to Mandvi Bharadwaj .

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Editors and Affiliations

  1. PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Wellington Street, Perth, 6000, Australia

    Frank T. Christiansen

  2. Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, 150-154 Batman Street, Melbourne VIC, 3000, Australia

    Brian D. Tait

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Bharadwaj, M., Mifsud, N.A., McCluskey, J. (2012). Detection and Characterisation of Alloreactive T Cells. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_18

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  • DOI: https://doi.org/10.1007/978-1-61779-842-9_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-841-2

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