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Studies on Carbohydrate Xenoantigens

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Xenotransplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 885))

Abstract

Naturally occurring and elicited anti-carbohydrate antibodies play a major role in immune responses to xenografts. The original obstacles associated with the Gal antigen have been largely resolved by the generation of knockout pigs. In contrast, much less is known about the nature and role of non-Gal carbohydrate antigens and the antibodies recognizing these. These antibodies can be identified and characterized by enzyme-linked immunosorbent assay. Furthermore, the biological significance of the non-Gal antigen(s) can be determined by expression of the relevant glycosyltransferase(s) by transfection and analyzed by antibody and/or lectin binding.

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Acknowledgment

This work was supported by funds from the National Health and Medical Research Council of Australia (NHMRC).

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Authors and Affiliations

  1. Department of Surgery (Austin Health), University of Melbourne, Heidelberg, VIC, Australia

    Dale Christiansen, Effie Mouhtouris, Paul A. Ramsland & Mauro S. Sandrin

  2. Centre for Immunology, Burnet Institute, Melbourne, VIC, Australia

    Paul A. Ramsland

  3. Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, VIC, Australia

    Paul A. Ramsland

Authors
  1. Dale Christiansen
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  2. Effie Mouhtouris
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  3. Paul A. Ramsland
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  4. Mauro S. Sandrin
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Corresponding author

Correspondence to Mauro S. Sandrin .

Editor information

Editors and Affiliations

  1. Bellvitge (IDIBELL), Hospital Duran i Reynals, Institut d'Investigació Biomèdica de, Gran Via de L’Hospitalet 199, L’Hospitalet de Llobregat, 08908, Barcelona, Spain

    Cristina Costa

  2. Servicio de Medicina Intensiva, Hospital Universitario de Bellvitge, Feixa Llarga s/n, L'Hospitalet de Llobregat, 08907, Barcelona, Spain

    Rafael Máñez

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Christiansen, D., Mouhtouris, E., Ramsland, P.A., Sandrin, M.S. (2012). Studies on Carbohydrate Xenoantigens. In: Costa, C., Máñez, R. (eds) Xenotransplantation. Methods in Molecular Biology, vol 885. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-845-0_4

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  • DOI: https://doi.org/10.1007/978-1-61779-845-0_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-844-3

  • Online ISBN: 978-1-61779-845-0

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