Abstract
This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.
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Acknowledgments
This work was supported by NIH grants RC4DK090891, UO1DK070251, and UO1DE020049. We thank Lauren Kadel and Andrew S. Potter for technical assistance with laser capture.
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© 2012 Springer Science+Business Media, LLC
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Potter, S.S., Brunskill, E.W. (2012). Laser Capture. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_19
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DOI: https://doi.org/10.1007/978-1-61779-851-1_19
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-850-4
Online ISBN: 978-1-61779-851-1
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