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Receptor Binding Techniques pp 261–302Cite as

Detection of mRNA Encoding Receptors by In Situ and Northern Hybridization

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 897))

Abstract

The ability to measure mRNA encoding protein is attractive, since, provided certain criteria are met, the investigator can be confident of highly selective detection. In addition, changes in mRNA expression as well as their precise cellular localization, provides the scientist with important information that may not be evident by the detection of the translated protein. Hence, the combined approach of assessing mRNA and protein expression will often allow a more precise hypothesis to be formulated. In the present chapter, we describe methods we have optimized for the detection of mRNA following extraction from tissue or cells (Northern hybridization) and the detection of specific mRNA transcripts within their synthesising cells (in situ hybridization).

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Author information

Authors and Affiliations

  1. Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, UK

    Alessandra P. Princivalle

  2. The British Heart Foundation, London, UK

    Rachel M. C. Parker

  3. Cellular and Molecular Neuropharmacology Research Group, Division of Neuroscience, Department of Pharmacology, The Medical School, University of Birmingham, Birmingham, UK

    Terri J. Dover

  4. Cellular and Molecular Neuropharmacology Research Group, Division of Neuroscience, Department of Pharmacology, University of Birmingham, The Medical School, Nottingham, UK

    Nicholas M. Barnes

Authors
  1. Alessandra P. Princivalle
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  2. Rachel M. C. Parker
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  3. Terri J. Dover
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  4. Nicholas M. Barnes
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Corresponding author

Correspondence to Nicholas M. Barnes .

Editor information

Editors and Affiliations

  1. Addenbrooke's Hospital, Box 110, Clinical Pharmacology Unit, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, United Kingdom

    Anthony P. Davenport

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Princivalle, A.P., Parker, R.M.C., Dover, T.J., Barnes, N.M. (2012). Detection of mRNA Encoding Receptors by In Situ and Northern Hybridization. In: Davenport, A. (eds) Receptor Binding Techniques. Methods in Molecular Biology, vol 897. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-909-9_13

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  • DOI: https://doi.org/10.1007/978-1-61779-909-9_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-908-2

  • Online ISBN: 978-1-61779-909-9

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