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Calcium Signaling Protocols pp 307–326Cite as

Compartmentalizing Genetically Encoded Calcium Sensors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 937))

Abstract

Within single cells there is a complex myriad of signaling which controls physiological process many of which are modulated, or signaled directly, by intracellular calcium ions. Understanding the exquisitely sensitive, and spatially restricted, changes in calcium has been of interest to the researcher for a number of years. Recent advances in this field have been driven by the development of genetically encoded calcium probes for detecting calcium changes within the cells specifically targeting organelles such as mitochondria, endoplasmic reticulum, and the nucleus. In this chapter the authors outline some of the available fluorescent probes, with particular emphasis on an endoplasmic reticulum targeted calcium biosensor in cell signaling studies with astrocytes, detailing experimental protocols and the interpretation of data from such probes.

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Authors and Affiliations

  1. Department of Physiology, The University of Melbourne, Melbourne, VIC, Australia

    David A. Williams, Mastura Monif & Kate L. Richardson

Authors
  1. David A. Williams
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  2. Mastura Monif
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  3. Kate L. Richardson
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Corresponding author

Correspondence to David A. Williams .

Editor information

Editors and Affiliations

  1. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    David G. Lambert

  2. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    Richard D. Rainbow

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Williams, D.A., Monif, M., Richardson, K.L. (2013). Compartmentalizing Genetically Encoded Calcium Sensors. In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_19

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  • DOI: https://doi.org/10.1007/978-1-62703-086-1_19

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-085-4

  • Online ISBN: 978-1-62703-086-1

  • eBook Packages: Springer Protocols

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