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Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo

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Book cover Stimulation and Inhibition of Neurons

Part of the book series: Neuromethods ((NM,volume 78))

Abstract

Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.

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Acknowledgments

The work reported here was supported by the National Health and Medical Research Council of Australia (Project Grants 229907, 480414, 350207). The juxtacellularly labelled neuron in Fig. 5 was filled by Dr. Ann Schreihofer in the laboratory of Dr. Patrice Guyenet. We thank them both for allowing us to use this image. The juxtacellularly labelled relaxin-3-positive neuron in Fig. 6 (panels c and d) was filled by Dr. Sherie Ma in the laboratory of Associate Professor Anthony Verberne.

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Authors and Affiliations

  1. Department of Medicine, Clinical Pharmacology and Therapeutics Unit, Austin Health, University of Melbourne, Heidelberg, VIC, Australia

    Anthony J. M. Verberne

  2. Cardiovascular Medicine, Physiology, and Centre for Neuroscience, Flinders University, Bedford Park, SA, Australia

    Ida J. Llewellyn-Smith

Authors
  1. Anthony J. M. Verberne
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  2. Ida J. Llewellyn-Smith
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Corresponding author

Correspondence to Anthony J. M. Verberne .

Editor information

Editors and Affiliations

  1. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Paul M. Pilowsky

  2. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Melissa M.J. Farnham

  3. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Angelina Y. Fong

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Verberne, A.J.M., Llewellyn-Smith, I.J. (2013). Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo. In: Pilowsky, P., Farnham, M., Fong, A. (eds) Stimulation and Inhibition of Neurons. Neuromethods, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-233-9_10

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  • DOI: https://doi.org/10.1007/978-1-62703-233-9_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-232-2

  • Online ISBN: 978-1-62703-233-9

  • eBook Packages: Springer Protocols

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