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Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patients with Acute Myeloid Leukemia

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Stem Cell Niche

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1035))

Abstract

Mounting evidences indicate that leukemic cells in patients with acute myeloid leukemia (AML) are derived from leukemia stem cells (LSC). In analogy to normal hematopoietic stem cells (HSC), LSC remain mostly dormant and are hence resistant to conventional chemotherapy. Residual, physiological HSC exist alongside with LSC, with heterogeneous dominance of LSC over HSC in individual patients. We have devised a flow cytometric method for the identification and separation of these two stem cell populations based on surface antigen markers such as CD34, CD38, lineage aberrant markers, and aldehyde dehydrogenase (ALDH) enzyme activity.

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Abbreviations

ALDH:

Aldehyde dehydrogenase

AM:

Aberrant marker

AML:

Acute myeloid leukemia

APC:

Allophycocyanin

FSC:

Forward scatter

FACS:

Fluorescence-activated cell sorting

FITC:

Fluorescein

HSC:

Hematopoietic stem cell

LSC:

Leukemia stem cells

MNC:

Mononuclear cells

NOD/SCIDNOD/SCID:

Non-Obese Diabetic/Severe Combined Immune Deficiency

NSG:

NOD/SCID/interleukin 2 receptor gammanull

PE:

Phycoerythrin

PI:

Propidium iodide

SSC:

Side scatter

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Authors and Affiliations

  1. Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany

    Van T. Hoang, Isabel Hoffmann, Karina Borowski, Abraham Zepeda-Moreno, Dan Ran, Eike C. Buss, Patrick Wuchter, Volker Eckstein & Anthony D. Ho

Authors
  1. Van T. Hoang
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  2. Isabel Hoffmann
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  3. Karina Borowski
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  4. Abraham Zepeda-Moreno
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  5. Dan Ran
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  6. Eike C. Buss
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  7. Patrick Wuchter
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  8. Volker Eckstein
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  9. Anthony D. Ho
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Editor information

Editors and Affiliations

  1. Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    Kursad Turksen

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Hoang, V.T. et al. (2013). Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patients with Acute Myeloid Leukemia. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 1035. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-508-8_19

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  • DOI: https://doi.org/10.1007/978-1-62703-508-8_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-507-1

  • Online ISBN: 978-1-62703-508-8

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