Abstract
Numerous methods have been developed for the formation of disulfide bonds in recombinant DNA-derived and chemically synthesized peptides and proteins, but only a few have found widespread acceptance. The choice of method(s) for formation of disulfide in synthetic peptides and proteins needs to be tailored for each individual polypeptide in such a way so that the reaction conditions are selective, efficient, and safe and give the maximum yield. Here we describe the sequential formation of three disulfide bonds regioselectively which has been optimized for the synthesis of two-chained, heterodimeric polypeptide members of the insulin–relaxin superfamily.
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Acknowledgements
Part of the work described herein was funded by NHMRC (Australia) project grants 350284 and 508995 to JDW. Studies at the FNI are also supported by the Victorian Government’s Operational Infrastructure Support Program.
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Shabanpoor, F., Hossain, M.A., Lin, F., Wade, J.D. (2013). Sequential Formation of Regioselective Disulfide Bonds in Synthetic Peptides with Multiple Disulfide Bonds. In: Jensen, K., Tofteng Shelton, P., Pedersen, S. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology, vol 1047. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-544-6_5
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DOI: https://doi.org/10.1007/978-1-62703-544-6_5
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-544-6
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