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Influence of Extracellular Environment on Electroporation Efficiency

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8th European Medical and Biological Engineering Conference (EMBEC 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 80))

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Abstract

In this work, the effects of extracellular environment, in terms of both electroporation (EP) medium and extracellular matrix (ECM), on EP efficiency were evaluated in a 3D in vitro model composed of HCC1954 cells cultured on hyaluronic acid (HA) hydrogels enriched with self-assembling peptides carrying IKVAV motifs. The results from 3D cultures were compared to those derived from cell in suspension and adherent cultures. EP was carried out by using either RPMI (high conductivity medium) and electroporation buffer (low conductivity medium) and applying 8 rectangular voltage pulses at 700 V (electric field strength 1000 V/cm with plate electrode with 7 mm gap). Collectively, our data highlighted that cell organization and the presence of ECM modulate local electrical properties, thus affecting EP efficiency.

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Acknowledgments

Authors are also grateful to Igea spa, Carpi (MO) Italy for the pulse generator loan. The research was made possible thanks to the networking of the ISEBTT, International Society for Electroporation-Based Technology and Treatments (http://www.electroporation.net/) and COST action CA16119 - In vitro 3-D total cell guidance and fitness (http://cost-cellfit.eu/).

Author information

Authors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, Padova University, via Marzolo, 5, 35131, Padua, Italy

    Bianca Bazzolo & Maria Teresa Conconi

  2. Department of Industrial Engineering, Padova University, via Marzolo, 9, 35131, Padua, Italy

    Monica Dettin & Annj Zamuner

  3. Department of Surgical Oncological and Gastroenterological Sciences DISCOG, Padova University, 35124, Padua, Italy

    Luca Giovanni Campana

  4. University of Insubria, via Dunant, 3, 21100, Varese, Italy

    Elisabetta Sieni

Authors
  1. Bianca Bazzolo
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  2. Maria Teresa Conconi
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  4. Annj Zamuner
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  5. Luca Giovanni Campana
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  6. Elisabetta Sieni
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Corresponding author

Correspondence to Elisabetta Sieni .

Editor information

Editors and Affiliations

  1. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Tomaz Jarm

  2. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Aleksandra Cvetkoska

  3. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Samo Mahnič-Kalamiza

  4. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Damijan Miklavcic

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Bazzolo, B., Conconi, M.T., Dettin, M., Zamuner, A., Campana, L.G., Sieni, E. (2021). Influence of Extracellular Environment on Electroporation Efficiency. In: Jarm, T., Cvetkoska, A., Mahnič-Kalamiza, S., Miklavcic, D. (eds) 8th European Medical and Biological Engineering Conference. EMBEC 2020. IFMBE Proceedings, vol 80. Springer, Cham. https://doi.org/10.1007/978-3-030-64610-3_75

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  • DOI: https://doi.org/10.1007/978-3-030-64610-3_75

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