Abstract
In Kluyveromyces marxianus, a thermotolerant yeast that has already been utilized for producing various useful materials, recent advances including complete genome sequencing and transcriptome analysis have provided valuable information on its physiological and metabolic characteristics including its capacity for assimilation of various sugars and tolerance to high temperatures, which are distinct from Saccharomyces cerevisiae. These prominent properties enable the development of high-temperature fermentation (HTF) technology for industrial applications. In addition, the yeast is able to integrate DNA fragments into its genome, which makes it easy to introduce foreign genes or gene sets into the genome. Moreover, DNA editing technology and simulation models are in place. Therefore, K. marxianus is a new platform in synthetic biology for producing useful materials.
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Acknowledgements
This study was supported by the Advanced Low Carbon Technology Research and Development Program, which was granted by the Japan Science and Technology Agency (JPMJAL1106) (MM, TK, and MY) and e-ASIA Joint Research Program, which was granted by Japan Science (JPMJSC16E5) (MM, TK, and MY) and Technology Agency, Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Agricultural Research Development Agency of Thailand and Ministry of Science and Technology of Laos, and partially supported by the Core to Core Program A. Advanced Research Networks, which was granted by the Japan Society for the Promotion of Science, the National Research Council of Thailand, Ministry of Science and Technology in Vietnam, National Univ. of Laos, Univ. of Brawijaya and Beuth Univ. of Applied Science Berlin (NL, MM, TK, and MY), and the Japan Society for the Promotion of Science, MEXT/JSPS Kakenhi (25250028 and 16H02485 to MY).
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Lertwattanasakul, N., Nurcholis, M., Rodrussamee, N., Kosaka, T., Murata, M., Yamada, M. (2022). Kluyveromyces marxianus as a Platform in Synthetic Biology for the Production of Useful Materials. In: Darvishi Harzevili, F. (eds) Synthetic Biology of Yeasts. Springer, Cham. https://doi.org/10.1007/978-3-030-89680-5_11
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