Abstract
Biosynthetic cargo enters the secretory pathway at the endoplasmic reticu-lum (ER) where it is properly folded before it becomes sorted and concentrated into transport carriers that move towards the Golgi complex. The basic protein machinery involved in bidirectional transport between the ER and the Golgi complex has long been identified and is fairly well characterized (Lee et al. 2004). The vesicular coat complex COPII mediates ER exit, while retrograde transport from the Golgi complex to the ER involves the function of the vesicular coat complex COPI. In the following paragraphs we focus on our current knowledge on the spatial and temporal organization of ER-to-Golgi transport at the molecular level.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Appenzeller-Herzog C, Hauri HP (2006) The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function. J Cell Sci 119: 2173–2183
Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, et al. (2006) Functional genomics reveals genes involved in protein secretion and Golgi organization. Nature 439: 604–607
Barlowe C, Orci L, Yeung T, Hosobuchi M, Hamamoto S, Salama N, Rexach MF, Ravazzola M, Amherdt M, Schekman R (1994) COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell 77:895–907
Ben-Tekaya H, Miura K, Pepperkok R, Hauri HP (2005) Live imaging of bidirectional traffic from the ERGIC. J Cell Sci 118: 357–367
Bentley M, Liang Y, Mullen K, Xu D, Sztul E, Hay JC (2006) SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. J Biol Chem 281:38825–38833
Bevis BJ, Hammond AT, Reinke CA, Glick BS (2002) De novo formation of transitional ER sites and Golgi structures in Pichia pastoris. Nat Cell Biol 4: 750–756
Bhattacharyya D, Glick BS (2007) Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization. Mol Biol Cell 18:839–849
Boutte Y, Vernhettes S, Satiat-Jeunemaitre B (2007) Involvement of the cytoskeleton in the secretory pathway and plasma membrane organisation of higher plant cells. Cell Biol Int 31: 649–654
Cai H, Yu S, Menon S, Cai Y, Lazarova D, Fu C, Reinisch K, Hay JC, Ferro-Novick S (2007) TRAPPI tethers COPII vesicles by binding the coat subunit Sec23. Nature 445: 941–994
Chen JL, Fucini RV, Lacomis L, Erdjument-Bromage H, Tempst P, Stamnes M (2005) Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles. J Cell Biol 169:383–389
Connerly PL, Esaki M, Montegna EA, Strongin DE, Levi S, Soderholm J, Glick BS (2005) Sec16 is a determinant of transitional ER organization. Curr Biol 15: 1439–1447
DaSilva LL, Snapp EL, Denecke J, Lippincott-Schwartz J, Hawes C, Brandizzi F (2004) Endoplasmic reticulum export sites and Golgi bodies behave as single mobile-secretory units in plant cells. Plant Cell 16: 1753–1771
Diao A, Frost L, Morohashi Y, Lowe M (2008) Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner. J Biol Chem 283:6957–6967
Dirac-Svejstrup AB, Shorter J, Waters MG, Warren G (2000) Phosphorylation of the vesicle-tethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments. J Cell Biol 150: 475–488
Forster R, Weiss M, Zimmermann T, Reynaud EG, Verissimo F, Stephens DJ, Pepperkok R (2006) Secretory cargo regulates the turnover of COPII subunits at single ER exit sites. Curr Biol 16: 173–179
Fromme JC, Schekman R (2005) COPII-coated vesicles: flexible enough for large cargo? Curr Opin Cell Biol 17: 345–352
Gilchrist A, Au CE, Hiding J, Bell AW, Fernandez-Rodriguez J, Lesimple S, Nagaya H, Roy L, Gosline SJ, Hallett M, et al (2006) Quantitative proteomics analysis of the secretory pathway. Cell 127: 1265–1281
Hammond AT, Glick BS (2000) Dynamics of transitional endoplasmic reticulum sites in vertebrate cells. Mol Biol Cell 11: 3013–3030
He CY (2007) Golgi biogenesis in simple eukaryotes. Cell Microbiol 9: 566–572
Iinuma T, Shiga A, Nakamoto K, O’Brien MB, Aridor M, Arimitsu N, Tagaya M, Tani K (2007) Mammalian Sec1 6/p250 plays a role in membrane traffic from the endoplasmic reticulum. J Biol Chem 282: 17632–17639
Jahn R, Scheller RH (2006) SNAREs engines for membrane fusion. Nat Rev Mol Cell Biol 7: 631–643
Kamena F, Spang A (2004) Tip20p prohibits back-fusion of COPII vesicles with the endoplasmic reticulum. Science 304: 286–289
Kano F, Tanaka AR, Yamauchi S, Kondo H, Murata M (2004) Cdc2 kinase-dependent disassembly of endoplasmic reticulum (ER) exit sites inhibits ER-to-Golgi vesicular transport during mitosis. Mol Biol Cell 15: 4289–4298
Lamandé SR, Bateman JF (1999) Procollagen folding and assembly: the role of endoplasmic reticulum enzymes and molecular chaperones. Semin Cell Dev Biol 10: 455–464
Lee MC, Miller EA, Goldberg J, Orci L, Schekman R (2004) Bi-directional proteintransport between the ER and Golgi. Annu Rev Cell Dev Biol 20: 87–123
Lee MC, Orci L, Hamamoto S, Futai E, Ravazzola M, Schekman R (2005) Sar 1p Nterminal helix initiates membrane curvature and completes the fission of a COPII vesicle. Cell 122:605–617
Lowe M, Barr FA (2007) Inheritance and biogenesis of organelles in the secretory pathway. Nat Rev Mol Cell Biol 8: 429–439
Mironov AA, MironovJr AA, Beznoussenko GV, Trucco A, Lupetti P, Smith JD, et al (2003) ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains. Dev Cell 5: 583–594
Moyer BD, Allan BB, Balch WE (2001) Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis-Golgi tethering. Traffic 2: 268–276
Orci L, Ravazzola M, Meda P, Holcomb C, Moore HP, Hicke L, Schekman R (1991) Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm. Proc Natl Acad Sci USA 88: 8611–8615
Palade G (1975) Intracellular aspects of the process of protein synthesis. Science 189: 347–358
Prescott AR, Farmaki T, Thomson C, James J, Paccaud JP, Tang BL, Hong W, Quinn M, Ponnambalam S, Lucocq J (2001) Evidence for prebudding arrest of ER export in animal cell mitosis and its role in generating Golgi partitioning intermediates. Traffic 2: 321–335
Preuss D, Mulholland J, Franzusoff A, Segev N, Botstein D (1992) Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy. Mol Biol Cell 3: 789–803
Presley JF, Cole NB, Schroer TA, Hirschberg K, Zaal KJ, Lippincott-Schwartz J (1997) ER-to-Golgi transport visualized in living cells. Nature 389: 81–85
Puthenveedu MA, Bachert C, Puri S, Lanni F, Linstedt AD (2006) GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution. Nat Cell Biol 8: 238–248
Robinson DG, Herranz MC, Bubeck J, Pepperkok R and Ritzenthaler Ch (2007) Membrane Dynamics in the Early Secretory Pathway. Crit Rev Plant Sci 26: 199–225
Rossanese OW, Soderholm J, Bevis BJ, Sears IB, O’Connor J, Williamson EK, Glick BS (1999) Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae. J Cell Biol 145: 69–81
Scales SJ, Pepperkok R, Kreis TE (1997) Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI. Cell 90: 1137–1148
Shima DT, Scales SJ, Kreis TE, Pepperkok R (1999) Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes. Curr Biol 9: 821–824
Shimoi W, Ezawa I, Nakamoto K, Uesaki S, Gabreski G, Aridor M, Yamamoto A, Nagahama M, Tagaya M, Tani K (2005) p125 is localized in endoplasmic reticulum exit sites and involved in their organization. J Biol Chem 280: 10141–10148
Simpson JC, Nilsson T, Pepperkok R (2006) Biogenesis of tubular ER-to-Golgi transport intermediates. Mol Biol Cell 17: 723–737
Stagg SM, Gurkan C, Fowler DM, LaPointe P, Foss TR, Potter CS, Carragher B, Balch WE (2006) Structure of the Sec13/31 COPII coat cage. Nature 439: 234–238 Erratum in: Nature 442: 840
Starkuviene V, Pepperkok R (2007) Differential requirements for ts-O45-G and procolla-gen biosynthetic transport. Traffic 8: 1035–1051
Stephens DY, Lin-Marq N, Pagano A, Pepperkok R, Paccaud J-P (2000) COPIcoated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. J Cell Sci 113:2177–2185
Stephens DJ, Pepperkok R (2001) Illuminating the secretory pathway: when do we need vesicles? J Cell Sci 114: 1053–1059
Stephens DJ (2003) De novo formation, fusion and fission of mammalian COPII-coated endoplasmic reticulum exit sites. EMBO Rep 4: 210–217
Xu D, Hay JC (2004) Reconstitution of COPII vesicle fusion to generate a pre-Golgi intermediate compartment. J Cell Biol 167: 997–1003
Zeuschner D, Geerts WJC, Van Donselaar E, Humbel BM, Slot JW, Koster AJ, Klumper-man J (2006) Immuno-electron tomography of ER exit sites reveals the existence of free COPII-coated transport carriers. Nat Cell Biol 8: 377–383
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer-Verlag/Wien
About this chapter
Cite this chapter
Verissimo, F., Pepperkok, R. (2008). ER-to-Golgi transport. In: Mironov, A.A., Pavelka, M. (eds) The Golgi Apparatus. Springer, Vienna. https://doi.org/10.1007/978-3-211-76310-0_20
Download citation
DOI: https://doi.org/10.1007/978-3-211-76310-0_20
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-76309-4
Online ISBN: 978-3-211-76310-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)