Abstract
Glycine-rich proteins (GRPs) with characteristic repetitive glycine stretches are ubiquitous in organisms of all Kingdoms and have distinct functions. It is believed that Gly-rich domains serve mainly for interactions with other proteins. Previously, we identified the tobacco UP30 gene as strongly upregulated by sulfur deficiency. It encodes a protein highly similar to cdiGRP which affects tobacco defense response by elevating cell wall callose deposits thus blocking systemic movement of viruses. The closest Arabidopsis thaliana homologue of UP30 is GRP-3 (At2g05520). Here we report that GRP-3 is induced in Arabidopsis seedlings in both sulfur and nitrogen deficiency conditions. The transgenic Arabidopsis plants with changed GRP-3 expression (either overexpressing or with silenced GRP-3) tend to have longer roots than the wild type, especially in the conditions of sulfur deficiency. The effect could be alleviated by the addition of auxin to the media. Moreover, we observed the increased callose deposition in both Arabidopsis lines suggesting its negative effects on shoot-to-root movement of auxins in nutrient deficient conditions.
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References
Dan H, Yang G, Zheng ZL (2007) A negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana. Plant Mol Biol 63:221–235
De Oliveira DE, Seurinck J, Inze D, Van Montagu M, Botterman J (1990) Differential expression of five Arabidopsis genes encoding glycine-rich proteins. Plant Cell 2:427–436
Gramegna G, Modesti V, Savatin DV, Sicilia F, Cervone F, De Lorenzo G (2016) GRP-3 and KAPP encoding interactors of WAK1 negatively affect defense responses induced by oligogalacturonides and local response to wounding. J Exp Bot 67:1715–1729
Jackson D (2015) Plasmodesmata spread their influence. F1000Prime Rep 7:25
Lewandowska M, Wawrzynska A, Kaminska J, Liszewska F, Sirko A (2005) Identification of novel proteins of Nicotiana tabacum regulated by short term sulfur starvation. In: Saito K, De Kok LJ, Stulen I, Hawkesford MJ, Schnug E, Sirso A, Rennenberg H (eds) Sulfur transport and assimilation in plants in the postgenomic era. Backhuys Publishers, Leiden, pp 153–156
Mangeon A, Junqueira RM, Sachetto-Martins G (2010) Functional diversity of the plant glycine-rich proteins superfamily. Plant Signal Behav 5:99–104
Park AR, Cho SK, Yun UJ, Jin MY, Lee SH, Sachetto-Martins G, Park OK (2001) Interaction of the Arabidopsis receptor protein kinase Wak1 with a glycine-rich protein AtGRP-3. J Biol Chem 276:26688–26693
Rahman A, Bannigan A, Sulaman W, Pechter P, Blancaflor EB, Baskin TI (2007) Auxin actin and growth of the Arabidopsis thaliana primary root. Plant J 50:514–528
Sachetto-Martins G, Franco LO, de Oliveira DE (2000) Plant glycine-rich proteins: a family or just proteins with a common motif? Biochim Biophys Acta 1492:1–14
Showalter AM (1993) Structure and function of plant cell wall proteins. Plant Cell 5:9–23
Ueki S, Citovsky V (2002) The systemic movement of a tobamovirus is inhibited by a cadmium-ion-induced glycine-rich protein. Nat Cell Biol 4:478–486
Ueki S, Citovsky V (2005) Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature. Proc Natl Acad Sci U S A 102:12089–12094
Wawrzynska A, Lewandowska M, Hawkesford MJ, Sirko A (2005) Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit. J Exp Bot 56:1575–1590
Acknowledgments
We thank to Dimitris Bouranis, Agricultural University of Athens, Greece for the protocol of callose staining. This work was supported by the grant from the Polish Ministry of Science and Higher Education (W16/7.PR/2011).
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Znój, A., Zientara-Rytter, K., Sęktas, P., Moniuszko, G., Sirko, A., Wawrzyńska, A. (2017). A Glycine-Rich Protein Encoded by Sulfur-Deficiency Induced Gene Is Involved in the Regulation of Callose Level and Root Elongation. In: De Kok, L., Hawkesford, M., Haneklaus, S., Schnug, E. (eds) Sulfur Metabolism in Higher Plants - Fundamental, Environmental and Agricultural Aspects. Proceedings of the International Plant Sulfur Workshop. Springer, Cham. https://doi.org/10.1007/978-3-319-56526-2_21
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DOI: https://doi.org/10.1007/978-3-319-56526-2_21
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