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Approaches for In Vitro Conservation of Woody Plants Germplasm

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Biodiversity and Conservation of Woody Plants

Part of the book series: Sustainable Development and Biodiversity ((SDEB,volume 17))

Abstract

The conservation of plant genetic diversity aims at preserving as much as possible extant species by using innovative and complementary approaches to guarantee the effectiveness of the safeguarding strategies and to face present problems and future threats. The development and implementation of different in vitro conservation techniques have provided improvements for the international exchange of germplasm, for the storage of different in vitro culture forms and for products generated by biotechnology. These methods are also a valuable alternative to relieve the need of large lands extensions, where reserve collections of trees belonging to many woody species are traditionally kept. This chapter provides information of several study cases, describes some useful protocols, and aims at presenting a brief overview of currently available techniques for in vitro conservation to medium- and long-term of woody plant germplasm.

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Acknowledgements

Research works on cryopreservation of citrus germplasm were funded by IPGRI projects (96/098 and 99/18), INIA project (RF98/033), and a postdoctoral fellowship to the first author.

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Authors and Affiliations

  1. Facultad de Ciencias Químicas, Universidad Veracruzana, Prol. Oriente 6, No. 1009, C.P. 94340, Orizaba, VER, Mexico

    María Teresa González-Arnao & Carlos A. Cruz-Cruz

  2. Instituto de Botánica del Nordeste (IBONE), Universidad Nacional del Nordeste (UNNE)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Agrarias, UNNE, Sargento Cabral No. 2131, C.C. 209, 3400, Corrientes, Argentina

    Natalia Dolce

  3. Departamento de Biotecnología-Biología Vegetal, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

    María Elena González-Benito

  4. Laboratorio de Biotecnología Forestal del Centro de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, INIFAP, Progreso No. 5, Delegación Coyoacán, C.P. 04010, Ciudad de México, Mexico

    Carlos R. Castillo Martínez

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  1. María Teresa González-Arnao
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  2. Natalia Dolce
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  3. María Elena González-Benito
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  4. Carlos R. Castillo Martínez
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  5. Carlos A. Cruz-Cruz
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Correspondence to María Teresa González-Arnao .

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Editors and Affiliations

  1. Formerly Forestry Consultant, Zobel Forestry Associates, New Paltz, New York, USA

    M. R. Ahuja

  2. University of Helsinki, Helsinki, Finland

    S. Mohan Jain

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González-Arnao, M.T., Dolce, N., González-Benito, M.E., Castillo Martínez, C.R., Cruz-Cruz, C.A. (2017). Approaches for In Vitro Conservation of Woody Plants Germplasm. In: Ahuja, M., Jain, S. (eds) Biodiversity and Conservation of Woody Plants. Sustainable Development and Biodiversity, vol 17. Springer, Cham. https://doi.org/10.1007/978-3-319-66426-2_13

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