Abstract
Endophytes (fungi and bacteria) have been known to live asymptomatically with plants throughout the different growth and developmental stages. Endophytic microbes provide an additional resource to the plant due to the presence of beneficial secondary metabolites, enzymes, and nutrients, which help the host to combat diverse arrays of stressful conditions of biotic and abiotic stresses. Extracellular enzymes are the product of microbial’s cell growth and perform its function outside the cell in many biological or environmental processes. In fact, certain category of enzymes namely, xylanases, hemicellulases, phytases, proteases, asparaginase, cellulases, pectinases, tyrosinase, gelatinase, chitinase, amylases, etc., are some of the key enzymes produced by endophytic bacteria and fungi. Most of these enzymes have been reported from endophytes living within medicinal or crop plants, whereas they are detected through agar-based methods. The current chapter aims to identify the sources, kinds of enzymes, and the perspectives for further studies in their application in endophytic-based extracellular enzymes resources.
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References
Akinsanya MA, Ting A, Goh JK, Lim SP (2016) Biodiversity, enzymatic and antimicrobial activities of bacterial endophytes in selected local medicinal plants. J Biomed Pharm Res 16:5(1)
Amirita A, Sindhu P, Swetha J, Vasanthi NS, Kannan KP (2012) Enumeration of endophytic fungi from medicinal plants and screening of extracellular enzymes. World J Sci Technol 2:13–19
Arachevaleta M, Bacon CW, Hoveland CS, Radcliffe DE (1989) Effect of the tall fescue endophyte on plant response to environmental stress. Agronom J 81:83–90
Ayob FW, Simarani K (2016) Endophytic filamentous fungi from a Catharanthus roseus: Identification and its hydrolytic enzymes. Saudi Pharm J 24:273–278
Bacon CW (1993) Abiotic stress tolerances (moisture, nutrients) and photosynthesis in endophyte-infected tall fescue. Agric Ecosys Environ 44:123–141
Bezerra JD, Santos MG, Svedese VM, Lima DM, Fernandes MJ, Paiva LM, Souza-Motta CM (2012) Richness of endophytic fungi isolated from Opuntia ficusindica Mill. (Cactaceae) and preliminary screening for enzyme production. World J Microbiol Biotechnol 28:1989–1995
Bezerra JD, Nascimento CC, Barbosa RD, da Silva DC, Svedese VM, Silva-Nogueira EB, Gomes BS, Paiva LM, Souza-Motta CM (2015) Endophytic fungi from medicinal plant Bauhinia forficata: diversity and biotechnological potential. Braz J Microbiol 46:49–57
Bhagat J, Kaur A, Chadha BS (2016) Single step purification of asparaginase from endophytic bacteria Pseudomonas oryzihabitans exhibiting high potential to reduce acrylamide in processed potato chips. Food Bioprod Process 99:222–230
Boer W, Folman LB, Summerbell RC, Boddy L (2005) Living in a fungal world: impact of fungi on soil bacterial niche development. FEMS Microbiol Rev 29:795–811
Burke RM, Cairney JWG (1997) Purification and characterization of a β-1,4-endoxylanase from the ericoid mycorrhizal fungus Hymenoscyphus ericae. New Phytol 35:345–352
Carrim AJ, Barbosa EC, Vieira JD (2006) Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham (Carobinha-do-campo). Braz Arch Biol Technol 49:353–359
Castro, GR, Mendez BS, Sin~eriz F (1993) Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR-41 in bath and continuous culture. J Chem Technol 50:289–294
Castro RA, Quecine MC, Lacava PT, Batista BD, Luvizotto DM, Marcon J et al (2014) Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. Springer Plus 3:382
Chathurdevi G, Gowrie SU (2016) Endophytic fungi isolated from medicinal plant—a source of potential bioactive metabolites. Int J Curr Pharm Res 8:50–56
Chen YT, Yuan Q, Shan LT, Lin MA, Cheng DQ, Li CY (2013) Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus. Oncol Lett 6:1787–1792
Chimwamurombe PM, Grönemeyer JL, Reinhold-Hurek B (2016) Isolation and characterization of culturable seed-associated bacterial endophytes from gnotobiotically grown Marama bean seedlings. FEMS Microbiol Ecol 1:92:fiw083
Chow Y, Ting AS (2015) Endophytic L-asparaginase producing fungi from plants associated with anticancer properties. JAR 6:869–876
Clay K, Holah J (1999) Fungal endophyte symbiosis and plant diversity in successional fields. Science 285(5434):1742–1744
Davis PE, Cohen DL, Whitaker A (1980) The production of α-amylase in batch and chemostat culture by Bacillus stearothermophilus. Antonie van Leeuwenhoek 46:391–398
De Almeida MN, Guimarães VM, Bischoff KM, Falkoski DL, Pereira OL, Gonçalves DS, de Rezende ST (2012) Cellulases and hemicellulases from endophytic acremonium species and its application on sugarcane bagasse hydrolysis. Appl Biochem Biotechnol 165:594–610
Ebrahiminezhad A, Rasoul-Amini S, Ghasemi Y (2011) L-asparaginase production by moderate halophilic bacteria isolated from Maharloo salt lake. Indian J Med Microbiol 51:307–311
Escudero N, Ferreira SR, Lopez-Moya F, Naranjo-Ortiz MA, Marin-Ortiz AI, Thornton CR, Lopez-Llorca LV (2016) Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia. Fungal Biol 120:572–585
Esteves AC, Saraiva M, Correia A, Alves A (2014) Botryo sphaeriales fungi produce extracellular enzymes with biotechnological potential. Can J Microbiol 60:332–342
Fillat Ú, Martín-Sampedro R, Macaya-Sanz D, Martín JA, Ibarra D, Martínez MJ, Eugenio ME (2016) Screening of eucalyptus wood endophytes for laccase activity. Process Biochem 51:589–598
Fouda AH, Hassan SE, Eid AM, Ewais EE (2015) Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.). Ann Agric Sci 60:95–104
Gazis R, Chaverri P (2010) Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru. Fungal Ecol 3:240–254
Giraud E, Gosselini L, Marin B, Parada JL, Raimbault M (1993) Purification and characterization of an extracellular amylase form Lactobacillus plantarum. J Appl Bacteriol 75:276–282
Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes. Soil Biol Biochem 37:395–412
Gupta V, Trivedi N, Kumar M, Reddy CR, Jha B (2013) Purification and characterization of exo-β-agarase from an endophytic marine bacterium and its catalytic potential in bioconversion of red algal cell wall polysaccharides into galactans. Biomass Bioenergy 28:290–298
Hallmann J, Qualt-Hallmann A, Mahaffee WF, Kloepper JW (1997) Bacterial endophytes in agricultural crops. Can J Microbiol 43:895–914
Harnpicharnchai P, Champreda V, Sornlake W, Eurwilaichitr L (2009) A thermotolerant beta-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars. Protein Exp Purific 67:61–69
Hoppe HG (1993) Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria. Handb Met Aquatic Microb Ecol 28:423–431
Idriss EE, Makarewicz O, Farouk A, Rosner K, Greiner R, Bochow H, Richter T, Borriss R (2002) Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiol 148:2097–2109
III HJ, Allison SD (2015) Cooperation, competition, and coalitions in enzyme-producing microbes: social evolution and nutrient depolymerization rates. The causes and consequences of microbial community structure 22:49
Isshiki A, Akimitsu K, Nishio K, Tsukamoto M, Yamamoto H (1997) Purification and characterization of an endopolygalacturonase from the rough lemon pathotype of Alternaria alternata, the cause of citrus brown spot disease. Physiol Mol Plant Pathol 51:155–167
Jamal MT, Mudarris MS (2010) Separation of YbdN bioactive protein from Bacillus subtilis isolated from the red sea algae Sargassum sp. with bioactivity against antibiotic resistant bacterial pathogens. Marine Sci 21:1
Joe MM, Devaraj S, Benson A, Sa T (2016) Isolation of phosphate solubilizing endophytic bacteria from Phyllanthus amarus Schum & Thonn: evaluation of plant growth promotion and antioxidant activity under salt stress. JARMAP 3:71–77
Joshi RD, Kulkarni NS (2016) Optimization studies on L-asparaginase production from endophytic bacteria. IJAR 2:624–629
Jurynelliz R-V, David P, Gloria O, Carlos R, Jesus-Bonilla D, Walleska (2016) Enzymatic and bacterial activity of fungal strains isolated from Alpinia zerumbet. Abstracts of Papers, 251st ACS National Meeting & Exposition, San Diego, CA, United States, March 13–17, CHED-1130
Kalyanasundaram I, Nagamuthu J, Srinivasan B, Pachayappan A, Muthukumarasamy S (2015) Production, purification and characterization of extracellular L-asparaginase from salt marsh fungal endophytes. World J Pharm Pharmaceut Sci 4:663–677
Kannan R, Damodaran T, Umamaheswari S (2015) Sodicity tolerant polyembryonic mango root stock plants: a putative role of endophytic bacteria. Afr J Biotechnol 14:350–359
Khan AL, Hussain J, Al-Harrasi A, Al-Rawahi A, Lee IJ (2015) Endophytic fungi: resource for gibberellins and crop abiotic stress resistance. Crit Rev Biotechnol 35:62–74
Khan AL, Al-Harrasi A, Al-Rawahi A, Al-Farsi Z, Al-Mamari A, Waqas M, Asaf S, Elyassi A, Mabood F, Shin JH, Lee IJ (2016) Endophytic fungi from frankincense tree improves host growth and produces extracellular enzymes and indole acetic acid. PLoS ONE 11:e0158207
Kharwar RNG, Surendra KKA, Mishra A (2010) A comparative study of endophytic and epiphytic fungal association with leaf of Eucalyptus citriodora Hook., and their antimicrobial activity. World J Microbiol Biotechnol 26:1941–1948
Leitão AL, Enguita FJ (2016) Gibberellins in Penicillium strains: Challenges for endophyte-plant host interactions under salinity stress. Microbiol Res 183:8–18
Leo VV, Passari AK, Joshi JB, Mishra VK, Uthandi S, Ramesh N, Gupta VK, Saikia R, Sonawane VC, Singh BP (2016) A novel triculture system (CC3) for simultaneous enzyme production and hydrolysis of common grasses through submerged fermentation. Front Microbiol 7. doi:10.3389/fmicb.2016.00447
Lindow SE, Brandl MT (2003) Microbiology of the phyllosphere. Appl Environ Microbiol 69:1875–1883
Lu F, Sun L, Lu Z, Bie X, Fang Y, Liu S (2007) Isolation and identification of an endophytic strain EJS-3 producing novel fibrinolytic enzymes. Curr Microbiol 54:435–439
Lupo S, Tiscornia S, Bettucci L (2001) Endophytic fungi from flowers, capsules and seeds of Eucalyptus globules. Rev Iberoam Micol 18:38–41
Luz JS, Silva RLO, Silveira EB, Cavalcante UMT (2006) Atividade enzimática de fungos endofíticos e efeito na promoção do crescimento de mudas de maracujazeiro-amarelo. Caatinga 19:128–134
Maria GL, Sridhar KR, Raviraja NS (2005) Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India. J Agric Technol 1:67–80
Mayerhofer MS, Fraser E, Kernaghan G (2015) Acid protease production in fungal root endophytes. Mycologia 107:1
Meijer M, Houbraken JAMP, Dalhuijsen S, Samson RA, Vries RP (2011) Growth and hydrolase profiles can be used as characteristics to distinguish Aspergillus Niger and other black aspergilla. Stud Mycol 69:19–30
Nongkhlaw FM, Joshi SR (2015) L-Asparaginase and antioxidant activity of endophytic bacteria associated with ethnomedicinal plants. Indian J Biotechnol 14:59–64
Nygren CM, Edqvist J, Elfstrand M, Heller G, Taylor AF (2007) Detection of extracellular protease activity in different species and genera of ectomycorrhizal fungi. Mycorrhiza 3:241–248
Patil MG, Pagare J, Patil SN, Sidhu AK (2015a) Extracellular enzymatic activities of endophytic fungi isolated from various medicinal plants. Int J Curr Microbiol App Sci 4:1035–1042
Patil MP, Patil RH, Maheshwari VL (2015b) Biological activities and identification of bioactive metabolite from endophytic Aspergillus flavus L7 isolated from Aegle marmelos. Curr Microbiol 71:39–48
Pereira SIA, Monteiro C, Vega AL, Castro PML (2016) Endophytic culturable bacteria colonizing Lavandula dentate L. plants: isolation, characterization and evaluation of their plant growth-promoting activities. Ecol Eng 87:91–96
Petrini O, Sieber TN, Toti L, Viret O (1993) Ecology, metabolite production, and substrate utilization in endophytic fungi. J Nat Toxins 1:185–196
Rabha AJ, Naglot A, Sharma GD, Gogoi HK, Veer V (2014) In vitro evaluation of antagonism of endophytic Colletotrichum gloeosporioides against potent fungal pathogens of Camellia sinensis. Indian J Microbiol 54:302–309
Roy S, Yasmin S, Ghosh S, Bhattacharya S, Banerjee D (2016) Anti-infective metabolites of a newly isolated Bacillus thuringiensis KL1 associated with kalmegh (Andrographis paniculata Nees.), a traditional medicinal herb. Microbiol Insigh 2016:1–7
Sáenz-de-Santamaría M, Guisantes JA, Martínez J (2016) Enzymatic activities of Alternaria alternata allergenic extracts and its major allergen (Alt a 1). Mycoses 49:288–292
Saikkonen K, Wäli P, Helander M, Faeth SH (2004) Evolution of endophyte–plant symbioses. Trends Plant Sci 9:275–280
Sakiyama CC, Paula EM, Pereira PC, Borges AC, Silva DO (2001) Characterization of pectin lyase produced by an endophytic strain isolated from coffee cherries. Lett Appl Microbiol 33:117–121
Schulz B, Boyle C, Draeger S, Römmert AK, Krohn K (2002) Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 106:996–1004
Sessitsch A, Hardoim P, Döring J, Weilharter A, Krause A, Woyke T, Mitter B, Hauberg-Lotte L, Friedrich F, Rahalkar M, Hurek T (2012) Functional characteristics of an endophyte community colonizing rice roots as revealed by metagenomic analysis. Mol Plant Microb Interac 25:28–36
Silva RLO, Luz JS, Silveira EB, Cavalcante UMT (2006) Fungos endofíticos em annona spp.: isolamento, caracterização enzimática e promoção do crescimento em mudas de pinha (annona squamosa L.). Acta Bot Bras 20:649–655
Sinsabaugh RS (1994) Enzymic analysis of microbial pattern and process. Biol Fertil Soils 17:69–74
Sorgatto M, Guimarães NCA, Zanoelo FF, Marques MR, Peixoto-Nogueira SC, Giannesi GG (2012) Purification and characterization of an extracellular xylanase produced by the endophytic fungus, Aspergillus terreus, grown in submerged fermentation. Afr J Biotechnol 11:8076–8084
Stamford TLM, Stamford NP, Coelho LCBB, Araujo JM (2001) Production and characterization of a thermostable α-amylase from Nocardiopsis sp. endophyte of yam bean. Bioresour Technol 76:137–141
Strong P J, Claus H (2011) Laccase: a review of its past and its future in bioremediation. Crit Rev Env Sci Tec 41(4)
Sturz AV, Christie BR, Nowak J (2000) Bacterial endophytes: potential role in developing sustainable systems of crop production. CRC Crit Rev Plant Sci 19:1–30
Sunitha VH, Devi DN, Srinivas C (2013) Extracellular enzymatic activity of endophytic fungal strains isolated from medicinal plants. WJAS 9:1–9
Suto M, Takebayashi M, Saito K, Tanaka M, Yokota A, Tomita F (2002) Endophytes as producers of xylanase. J Biosci Bioeng 93:88–90
Tan RX, Zou WX (2001) Endophytes: a rich source of functional metabolites. Nat Prod Rep 18:448–449
Traving SJ, Thygesen UH, Riemann L, Stedmon CA (2015) A model of extracellular enzymes in free-living microbes: which strategy pays off? Appl Environ Microbiol 81:7385–7393
Verma SC, Ladha JK, Tripathi AK (2001) Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep-water rice. J Biotechnol 91:127–141
Vijayalakshmi R, Kairunnisa K, Sivvaswamy SN, Dharan SS, Natarajan S (2016) Enzyme production and antimicrobial activity of endophytic bacteria isolated from medicinal plants. Indian J Sci Technol 9(14)
Wallenstein MD, Weintraub MN (2008) Emerging tools for measuring and modeling the in situ activity of soil extracellular enzymes. Soil Biol Biochem 40:2098–2106
Ward OP, Qin WM, Dhanjoon J, Ye J, Singh A (2005) Physiology and biotechnology of Aspergillus. Adv Appl Microbial 58:1–75
Wei DL, Chang SC, Wei YH, Lin YW, Chuang CL, Jong SC (1992) Production of cellulolytic enzymes from the Xylaria and Hypoxylon species of xylariaceae. World J Microbiol Biotechnol 8:141–146
White JF, Torres MS (2010) Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection? Physiol Plant 138:440–446
Wingender J, Neu TR, Flemming HC (2012) Microbial extracellular polymeric substances: characterization, structure and function. Springer Science & Business Media
Wipusaree N, Sihanonth P, Piapukiew J, Sangvanich P, Karnchanatat A (2011) Purification and characterization of a xylanase from the endophytic fungus Alternaria alternata isolated from the Thai medicinal plant, Croton oblongifolius roxb. Afr J Microbiol 5:5697–5712
Xiong XQ, Liao HD, Ma JS, Liu XM, Zhang LY, Shi XW (2013) Isolation of a rice endophytic bacterium, Pantoea sp. Sd-1, with ligninolytic activity and characterization of its rice straw degradation ability. Lett Appl Microbiol 58:123–129
Yang H, Wu H, Wang X, Cui Z, Li Y (2011) Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases. Bioresour Technol 102:3546–3550
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Khan, A.L., Shahzad, R., Al-Harrasi, A., Lee, IJ. (2017). Endophytic Microbes: A Resource for Producing Extracellular Enzymes. In: Maheshwari, D., Annapurna, K. (eds) Endophytes: Crop Productivity and Protection. Sustainable Development and Biodiversity, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-319-66544-3_5
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