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Functional Genomics Approach Towards Dissecting Out Abiotic Stress Tolerance Trait in Plants

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Genetic Enhancement of Crops for Tolerance to Abiotic Stress: Mechanisms and Approaches, Vol. I

Part of the book series: Sustainable Development and Biodiversity ((SDEB,volume 20))

Abstract

Plant functional genomics has revolutionized not only the methodologies for identification and elucidation of key genes’ function but also in designing strategies for improving tolerance towards abiotic stresses . Leveraging various approaches has demonstrated the robustness and versatility in their application to study gene/genome function and engineering abiotic stress tolerance in plants. With the emergence of novel high throughput technologies in this area, functional genomics can contribute immensely in understanding the gene regulatory networks operating under stress, thereby benefiting crop improvement programs. This chapter provides recent findings in the field of functional genomics , thus offering several efficacious methodologies such as next generation sequencing, genome -wide hybridization, gene-inactivation and genome -editing-based strategies in addition to metabolite analysis for discovery as well as validation of the candidate genes. Further, methodologies such as gene expression microarrays , insertional mutagenesis , map-based cloning and various genomic-assisted methods are evaluated critically and discussed in the light of integration of the information obtained through functional genomics with practical application in crop breeding .

Rohit Joshi and Brijesh K. Gupta have equally contributed to this work.

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Acknowledgements

Research in our lab is supported by funds from the Department of Biotechnology, Council of Scientific and Industrial Research, Government of India, internal grants of ICGEB, and Bioseed Research India. RJ acknowledges the Start-Up research grant (Young Scientist) from the Science and Engineering Research Board and Dr. D. S. Kothari Fellowship from University Grants Commission, Government of India.

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Authors and Affiliations

  1. Plant Stress Biology, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Rohit Joshi, Brijesh K. Gupta & Sneh L. Singla-Pareek

  2. Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Ashwani Pareek

  3. Plant Molecular Biology and Biotechnology Laboratory, University of Melbourne, Parkville, VIC, 3010, Australia

    Mohan B. Singh

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  1. Rohit Joshi
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  5. Sneh L. Singla-Pareek
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  1. Department of Botany, Hansraj College, University of Delhi, Delhi, India

    Vijay Rani Rajpal

  2. CIMMYT Headquarters, El Batán, Veracruz, Mexico

    Deepmala Sehgal

  3. Vinoba Bhave University, Hazaribag, Jharkhand, India

    Avinash Kumar

  4. Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India

    S.N. Raina

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Joshi, R., Gupta, B.K., Pareek, A., Singh, M.B., Singla-Pareek, S.L. (2019). Functional Genomics Approach Towards Dissecting Out Abiotic Stress Tolerance Trait in Plants. In: Rajpal, V., Sehgal, D., Kumar, A., Raina, S. (eds) Genetic Enhancement of Crops for Tolerance to Abiotic Stress: Mechanisms and Approaches, Vol. I. Sustainable Development and Biodiversity, vol 20. Springer, Cham. https://doi.org/10.1007/978-3-319-91956-0_1

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