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Strategies and Tools for Sequencing of the Sesame Genome

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The Sesame Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

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Abstract

The invention of Sanger sequencing initiated the genome era. The development and application of next-generation sequencing (NGS) technologies facilitated genome sequencing in organisms. Here we introduce the main technologies including Sanger sequencing, Roche-454 massive parallel pyrosequencing, Solexa/Illumina sequencing, ABI SOLiD platform, PacBio SMRT platform, HiC sequencing, Oxford nanopore sequencing, and Bionano sequencing platform, as mostly are applied in the Sesame Genome Project (SGP). The characteristics of each technology are briefly discussed. For the SGP, the hybrid sequencing strategies involving the first generation, NGS, and the third generation sequencing technologies are applied to assemble the fine genome map with high accuracy and completeness. Combined with the genome size estimation and genome characters of sesame, the efficient sequencing strategies are elucidated in this section.

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Author information

Authors and Affiliations

  1. Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, P. R. China

    Hongmei Miao & Haiyang Zhang

  2. TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, P. R. China

    Yamin Sun & Lei Wang

Authors
  1. Hongmei Miao
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  2. Yamin Sun
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  3. Lei Wang
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  4. Haiyang Zhang
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Corresponding author

Correspondence to Haiyang Zhang .

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Editors and Affiliations

  1. Henan Academy of Agricultural Sciences, Henan Sesame Research Center, Zhengzhou, China

    Hongmei Miao

  2. Henan Academy of Agricultural Sciences, Henan Sesame Research Center, Zhengzhou, China

    Haiyang Zhang

  3. ICAR-National Institute for Plant Biotechnology, New Delhi, India

    Chittaranjan Kole

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Miao, H., Sun, Y., Wang, L., Zhang, H. (2021). Strategies and Tools for Sequencing of the Sesame Genome. In: Miao, H., Zhang, H., Kole, C. (eds) The Sesame Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-98098-0_12

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