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Digital Holographic Microscopy Working with a Partially Spatial Coherent Source

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Coherent Light Microscopy

Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 46))

Abstract

We investigate the use of partially spatial coherent illuminations for digital holographic microscopes (DHMs) working in transmission. Depending on the application requirements, the sources are made from a spatially filtered LED or from a decorrelated laser beam. The benefits gained with those sources are indicated. A major advantage is the drastic reduction of the speckle noise making possible high image quality and the proper emulation of phase contrast modes such as differential interference contrast (DIC) . For biomedical applications, the DHMs are coupled with fluorescence sources to achieve multimodal diagnostics. Several implementations of biomedical applications where digital holography is a significant improvement are described. With a fast DHM permitting the analysis of dynamical phenomena, several applications in fluid physics and biomedical applications are also provided.

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Authors and Affiliations

  1. Microgravity Research Center, Université Libre de Bruxelles, 50 Av. F. Roosevelt, CP 165/62, B-1050, Brussels, Belgium

    Frank Dubois

  2. Microgravity Research Center, Université Libre de Bruxelles, B-1050, Brussels, Belgium

    Catherine Yourassowsky, Natacha Callens, Christophe Minetti & Patrick Queeckers

  3. Laboratoire de Spectrométrie Physique, Université Joseph Fourier – Grenoble, Grenoble, France

    Thomas Podgorski

  4. RUBIO – IRIBHM, IBMM – ULB Aéropole – CP 300, Université Libre de Bruxelles, B-6041, Charleroi – Gosselies, Belgium

    Annick Brandenburger

Authors
  1. Frank Dubois
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  2. Catherine Yourassowsky
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  3. Natacha Callens
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  4. Christophe Minetti
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  5. Patrick Queeckers
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  6. Thomas Podgorski
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  7. Annick Brandenburger
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Correspondence to Frank Dubois .

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Editors and Affiliations

  1. Ist. Nazionale di Ottica Applicata, CNR, Via Campi Flegrei 34, Pozzuoli, 80078, Napoli, Italy

    Pietro Ferraro

  2. Dept. Biomedical Engineering, Duke University, Durham, 27708-0292, North Carolina, USA

    Adam Wax

  3. Dept. Engineering, Bar-Ilan University, Ramat Gan, 52900, Israel

    Zeev Zalevsky

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Dubois, F. et al. (2011). Digital Holographic Microscopy Working with a Partially Spatial Coherent Source. In: Ferraro, P., Wax, A., Zalevsky, Z. (eds) Coherent Light Microscopy. Springer Series in Surface Sciences, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15813-1_2

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