Abstract
Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
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References
Blout, E. R.: In Optical rotatory dispersion, by C. Djerassi, Chap. 17. New York: McGraw-Hill 1960.
- Blout, E. R., C. de Lozé, S. M. Bloom, G. D. Fasman: J. Am. Chem. Soc. 82, 3787 (1960).
- Bradbury, E. M., A. R. Downie, A. Elliott and W. E. Hanby: Nature 187, 321 (1960).
Cohen, C.: Nature 175, 129 (1955).
- Coombes, J. D., E. Katchalski and P. Doty: Nature 185, 534 (1960).
Djerassi, C: Optical rotatory dispersion. New York: McGraw-Hill 1960.
- Doty, P., J. H. Bradbury and A. M. Holtzer: J. Am. Chem. Soc. 78,947 (1956).
- Doty, P., and J. T. Yang: J. Am. Chem. Soc. 78, 498 (1956).
- Doty, P., and R. D. Lundberg: Proc. U.S. Nat. Acad. Sci. 43, 213 (1957).
- Doty, P.: Proceedings of the IVth International Congress of Biochemistry, Vienna, Vol. 9, p. 9. London: Pergamon Press 1959.
- Downie, A. R., A. Elliott, W. E. Hanby and B. R. Malcolm: Proc. Roy. Soc. A 242, 325 (1957).
Fasman, G. D., and E. R. Blout: J. Am. Chem. Soc. 82, 2262 (1960).
- Fitts, D. D., and J. G. Kirkwood: Proc. U.S. Nat. Acad. Sci. 42, 33 (1956); 43, 1046 (1957);
- J. Am. Chem. Soc. 78, 2650 (1956)
- Foss, J. G.: Biochem. Biophys. Acta 43, 300 (1960).
- Foss, J. G., and J. A. Schellman: J. Phys. Chem. 63, 2007 (1960).
Goodman, M., E. E. Schmidt and D. Yphantis: J. Am. Chem. Soc. 82, 3483 (1960).
Harrington, W. F., and J. A. Schellman: Compt. rend. trav. lab. Carlsberg Ser Chim. 30, 21 (1956).
- Harrington, W. F., and M. Sela: Biochim. Biophys. Acta 27, 24 (1958).
- Heller, W.: Polarimetry in A. Weissberger (ed.), Physical methods of organic chemistry, 3rd ed., Vol. 1, Part 3, Chap. 33. New York: Interscience Publishers, Inc. 1949.
- Huggins, M. L.: J. Am. Chem. Soc. 74, 3963 (1952).
Imahori, K.: Biochim. Biophys. Acta 37, 336 (1960).
- Imahori, K., A. Yoshida and H. Hashizume: Biochim. Biophys. Acta 45, 380 (1960).
Jirgensons, B.: Arch. Biochem. Biophys. 74, 70 (1958); 78, 227, 235 (1958); 89, 48 (1960) and other earlier papers cited in these references.
Katchalski, E. : Proceedings of the IVth International Congress of Biochemistry, Vienna, Vol. 9, p. 21. London: Pergamon Press 1959.
- Karlson, R. H., K. S. Norland, G. D. Fasman and E. R. Blout: J. Am. Chem. Soc. 82, 2268 (1960).
- Kauzmann, W.: Ann. Rev. Phys. Chem. 8, 413 (1957);
- Adv. Protein Chem. XIV, 1 (1959)
- Kendrew, J. C., R. E. Dickerson, B. E. Strandberg, R. G. Hart, D. R. Davies, D. C. Phillips and V. C. Shore : Nature 185, 422 (1960).
- Klyne, W., and A. C. Parker: Optical rotatory dispersion in A. Weissberger (ed.), Physical methods of organic chemistry, 3rd ed. Vol. 1, Part III, Chapt. 34. New York: Interscience Publishers, Inc. 1960.
- Kortüm, G., and M. Kortüm-Seiler: Mod. Meth. Plant Analysis 1, 278 (1956).
- Kuhn, W. : Ann. Rev. Phys. Chem. 9, 417 (1958).
Leach, S. J.: Rev. Pure App. Chem. (Australia) 9, 33 (1959).
- Linderstrom-Lang, K. U., and J. A. Schellman: Biochem. Biophys. Acta 15, 156 (1954).
- Lowry, T. M.: Optical rotatory power. London: Longman, Green & Co. Ltd. 1935.
Malcolm, B. R., and A. E. Elliott: J. Sci. Instr. 34, 48 (1957).
- Mitchell, S.: J. Sci. Instr. 34,89 (1957).
- Moffitt, W. : J. Chem. Phys. 25,467 (1956).
- Moffitt, W., and J. T. Yang: Proc. U.S. Nat. Acad. Sci. 42, 596 (1956).
- Moffitt, W., D. D. Fitts and J. G. Kirkwood: Proc. U.S. Nat. Acad. Sci. 43, 723 (1957).
Robinson, C., and M. J. Bott: Nature 168, 325 (1951).
- Rudolph, H.: J. Opt. Sci. Am. 45, 50 (1955).
Schellman, J. A. : Compt. Rend. trav. lab. Carlsberg, Ser. chim. 30, 363, 395, 415, 429, 439,450 (1958 a).
- Schellman, C. G., and J. A. Schellman : Compt. rend. trav. lab. Carlsberg, Ser chim. 30, 465 (1958b).
- Schellman, J. A. : J. Phys. Chem. 62,1485 (1958c).
- Schellman, J. A., and C. G. Schellman: J. Polymer Sci. 49, 129 (1961)
- Shooter, E. M.: Progr. in Biophys. and Biophys. Chem. 10, 196 (1960).
- Steinberg, I. Z., M. Sela, W. F. Harrington, A. Berger and E. Katchalski: J. Am. Chem. Soc. 82, 5263 (1960).
Tanford, C., P. K. De, and V. G. Taggart: J. Am. Chem. Soc. 82, 6028 (1960).
Yang, J. T., and P. Doty: J. Am. Chem. Soc. 79, 761 (1957).
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Woods, E.F., O’;Donnell, I.J. (1962). Optical Rotatory Dispersion. Its Application to Protein Conformation. In: Biemann, K., et al. Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse. Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45993-1_11
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