Abstract
This chapter describes two procedures for the isolation of chromosomal DNA from E. coli. These procedures can be used for most gram-negative and gram-positive bacteria or modified to isolate DNA from organisms other than bacteria.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Crouse J and Amorese D 1987 Ethanol precipitation: Ammonium acetate as an alter-native to sodium acetate. BRL Focus 9 (2): 3–5
Hodge R 1994 In: Protocols for nucleic acid analysis by nonradioactive probes. Method in Molecular Biology Volume 28. Ed. Isaac PG. Humana Press. Totowa, New Jersey.
Jhingan AK 1992 A novel technology for DNA isolation. Methods in Molecular and Cel-lular Biology 3:1–22.
MurrayMG and Thompson WF 1980 Rapid isolation of high molecular weight DNA. Nucleic Acids Res. 8:43221–4325
Perbal BV 1898 A Practical guide to molecular cloning. 2nd edition. John Wiley & Sons Inc
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Surzycki, S. (2000). Preparation of Genomic DNA from Bacteria. In: Basic Techniques in Molecular Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56968-5_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-56968-5_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66678-3
Online ISBN: 978-3-642-56968-5
eBook Packages: Springer Book Archive