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Microfluidic Filtration Chip for DNA Extraction and Concentration

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Microreaction Technology: Industrial Prospects

Abstract

In the last few years there has been tremendous interest in developing a complete biomedical/biochemical intelligent microsystem for extraction, concentration, amplification, analysis and processing of DNA. This paper presents multi-disciplinary computational tool, CFD-ACE+MEMS, for simulation and design of microfluidic bioMEMS. It is demonstrated on 3D high-fidelity simulations in microfluidic channels and on a complex DNA filtration chip.

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References

  1. Jacobsen C.J., et al., “Microchip Capillary Electrophoresis with an Integrated Postcolumn Separation,” Anal. Chem. 66, p. 3472, 1994.

    Article  Google Scholar 

  2. Wooley A.T. and Mathies R.A., “Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips,” Anal. Chem. 67, p. 3676, 1995.

    Article  Google Scholar 

  3. Wooley A.T., et al, “Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device,” Anal. Chem. 68, p. 4081, 1996.

    Google Scholar 

  4. Northrup M.A., “A Miniature Analytical Instrument for Nucleic Acids Based on Micromachined Silicon Reaction Chambers,” Anal. Chem. 70, p. 918, 1998.

    Article  CAS  Google Scholar 

  5. Waters L.C. et al. “Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip,” Anal. Chem. 70, p. 5172, 1998.

    Article  CAS  Google Scholar 

  6. Christel L.A. et al “High Aspect Ratio Silicon Microstructures for Nucleic Acid Extraction,” Solid State Sensors and Actuators Workshop, Hilton Head, SC, June 7–11, 1998

    Google Scholar 

  7. Vo-Dinh et al. “DNA Biochip Using a Phototransistor Integrated Circuit,” Anal.Chem. 71, p. 358, 1999.

    Article  CAS  Google Scholar 

  8. Olson W. K. “Computational Approaches to Nucleic Acid Structure,” In Computational Biomolecular Structures, ed. By Soumpasis D.M. and Jovin T.M. Springer-Verlag, 1993.

    Google Scholar 

  9. Robinson B.H. and Drobny G.P. “Site Specific Dynamics in DNA: Theory,” Ann Rev. Biophys. Biomol. Struct. V24, p. 523, 1995.

    Article  CAS  Google Scholar 

  10. Long D. Viovy J.L., and Ajdari A., “Simultaneous action of Electric Fields and Nonelectric Forces on a Polyelectrolyte,” Phys. Rev. Lett. 76, p. 3858, 1996

    Article  CAS  Google Scholar 

  11. Wetmur J.G., “DNA Probes: Applications of the Principles of Nucleic Acid Hybridization,” Crit. Rev. in Biochem. And Mol. Biol. 26 (3/4), p. 227, 1991.

    Article  CAS  Google Scholar 

  12. Ermakov S.V., et al., “Computer Simulation of Electrokinetic Transport in Microfabricated Channel Structures,” Anal. Chem. 70, p. 4494, 1998.

    Article  CAS  Google Scholar 

  13. Giridharan M.G., et al., “Computational Simulation of Microfluidics, Electrokinetics, and Particle Transport in Bilogical MEMS Devices,” Symp. On Design, Test and Microfabrication of MEMS/MOEMS, Paris, March 30, 1999.

    Google Scholar 

  14. Yang, H.Q. and Przekwas, A.J., “Computational Modeling of Microfluid Devices with Free Surface Handling,” MSM-98, Santa Clara, CA, 1998.

    Google Scholar 

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© 2000 Springer-Verlag Berlin Heidelberg

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Przekwas, A., Wang, D., Makhijani, V.B., Przekwas, A.J. (2000). Microfluidic Filtration Chip for DNA Extraction and Concentration. In: Ehrfeld, W. (eds) Microreaction Technology: Industrial Prospects. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59738-1_51

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  • DOI: https://doi.org/10.1007/978-3-642-59738-1_51

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64104-6

  • Online ISBN: 978-3-642-59738-1

  • eBook Packages: Springer Book Archive

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