Abstract
Coliphage λ is a temperate virus. A fraction of λ infected bacterial cells survive, and some of the survivors become lysogens. Lysogens are bacteria that have stably acquired two new properties, i.e., immunity to infection by a further challenge of virus and the capacity to produce infective virus. The new properties reflect the presence of λ prophage, the genome of λ virus as it occurs in lysogens. Lysogenization, the establishment of the prophage-containing state, was recognized early to depend on two potentially independent processes. First, lytic growth of the virus must be prevented or the infected cell will die. This process is accomplished primarily by λ repressor whose molecular basis of action and role in lysogeny are described in detail in recent reviews (Pirrotta, 1976); Ptashne et al., 1976). Second, the prophage must be made to replicate in parity with the host genome. For lysogens of phage λ, this is accomplished by the insertion of the entire viral genome into the host genome, forming a single continuous chromosome in which the viral DNA is replicated as part of the host genome.
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References
Adhya, S., Gottesman, M., De Crombrugghe, B., Court, D.: Transcription termination regulates gene expression. In: RNA Polymerase. New York: Cold Spring Harbor Laboratory 1976, pp. 719–730
Ausubel, F.: Radiochemical purification of bacteriophage lambda integrase. Nature 247, 152–154 (1974)
Ausubel, F., Voynow, P., Signer, E., Mis try, J.: Purification of proteins determined by two nonessential genes in lambda. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 395–405
Botchan, P.: An electron microscopic comparison of transcription on linear and superhelical DNA. J. Mol. Biol. 105, 161–176 (1976)
Brack, C., Bickle, T.A., Yuan, R.: The relation of single-stranded regions in bacteriophage PM2 supercoiled DNA to the early melting sequences. J. Mol. Biol. 96, 693–702 (1975)
Campbell, A.M.: Episomes. Adv. Genet. 11, 101–145 (1962)
Campbell, A.M.: Episomes. New York: Harper & Row 1969, pp. 81–94, 99–113
Campbell, A.M.: How viruses insert their DNA into the DNA of the host cell. Sci. Am. 235, 103–113 (1976a)
Campbell, A.M.: Significance of constitutive integrase synthesis. Proc. Natl. Acad. Sci. U.S.A. 73, 887–890 (1976b)
Campbell, A., Hefferman, L., Hu, S., Szybalski, W.: The integrase promoter of bacteriophage lambda. In: Plasmids, DNA Insertion Elements and Episomes.Shapiro, J., Bukhari, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory, 1977, pp. 375–379
Chung, S., Echols, H.: Positive regulation of integrative recombination by the ell and cIII genes of bacteriophage X. Virology 79, 312–319 (1977)
Court, D., Adhya, S., Nash, H., Enquist, L.: The phage X integration protein (Int) is subject to control by the ell and cIII gene products. In: Plasmids, DNA Insertion Elements and Episomes. Shapiro, J., Bukhari, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory 1977, pp. 389–394
Crick, F.H.C., Klug, A.: Kinky helix. Nature 255, 530–533 (1975)
Davis, R.W., Parkinson, J.S.: Deletion mutants of bacteriophage lambda. III. Physical structure of att. J. Mol. Biol. 56, 403–423 (1971)
Davies, R.W., Dove, W.F., Inokuchi, H., Lehman, J.F., Roehrdanz, R.L.: Regulation of X prophage excision by the transcriptional state of DNA. Nature [New Biol.] 238, 43–45 (1972)
Dickson, R.C., Abelson, J., Barnes, W.M., Reznikoff, W.S.: Genetic regulation: The Lac control region. Science 187, 27–35 (1975)
Doerfler, W.: Integration of viral DNA into the host genome. Curr. Top. Microbiol. Immunol. 71, 1–78 (1975)
Dove, W.F.: An energy-level hypothesis for X prophage insertion and excision. J. Mol. Biol. 47, 585–589 (1970)
Echols, H.: Integrative and excisive recombination by bacteriophage X: Evidence for an excision-specific recombination protein. J. Mol. Biol. 47, 575–583 (1970)
Echols, H.: Constitutive integrative recombination by bacteriophage X. Virology 64, 557–559 (1975)
Echols, H., Green, L.: Establishment and maintenance of repression by bacteriophage lambda: The role of the el, cII, and cIII proteins. Proc. Natl. Acad. Sci. U.S.A. 68, 2190–2194 (1971)
Echols, H., Gingery, R., Moore, L.: Integrative recombination function of bacteriophage X: Evidence for a site-specific recombination enzyme. J. Mol. Biol. 34, 251–260 (1968)
Echols, H., Chung, S., Green, L.: Site-specific recombination: Genes and regulation. In: Mechanisms in Recombination. Grell, R.F. (ed.). New York: Plenum Press 1974, pp. 69–77
Eisen, H., Brachet, P., Pereita da Silva, L., Jacob, F.: Regulation of repressor expression in X. Proc. Natl. Acad. Sci. U.S.A. 66, 855–862 (1970)
Engler, J., Inman, R.B.: Site-specific recombination in bacteriophage lambda. J. Mol. Biol. 113, 385–400 (1977)
Enquist, L.W., Skalka, A.: Replication of bacteriophage X DNA dependent on the function of host and viral genes. J. Mol. Biol. 75, 185–212 (1973)
Enquist, L.W., Weisberg, R.A.: The red plaque test: A rapid method for identification of excision defective variants of bacteriophage lambda. Virology 72, 147–153 (1976)
Enquist, L.W., Weisberg, R.A.: A genetic analysis of the att-int-xis region of coliphage lambda. J. Mol. Biol. III, 97–120 (1977a)
Enquist, L.W., Weisberg, R.A.: Flexibility in attachment site recognition by X integrase. In: Plasmids, DNA Insertion Elements and Episomes. Shapiro, J., Bukhari, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory 1977b, pp. 343–348
Fiandt, M., Gottesman, M.E., Shulman, M.J., Szybalski, E.H., Szybalski, W., Weisberg, R.A.: Physical mapping of coliphage Xatt2. Virology 72, 6–12 (1976)
Folkmanis, A., Freifelder, D.: Studies on lysogeny in Escherichia coli with bacteriophage. X. Physical observation of the insertion process. J. Mol. Biol. 65, 63–73 (1972)
Freif elder, D., Levine, E.E.: Requirement for transcription in the neighborhood of the phage attachment region for lysogenization of Escherichia coli by bacteriophage X. J. Mol. Biol. 74, 729–733 (1973)
Freifelder, D., Meselson, M.: Topological relationship of prophage X to the bacterial chromosome in lysogenic cells. Proc. Natl. Acad. Sci. U.S.A. 65, 200–205 (1970)
Freifelder, D., Kirschner, I., Goldstein, R., Baran, N.: Physical study of prophage excision and curing of X prophage from lysogenic Escherichia coli. J. Mol. Biol. 74, 703–720 (1973)
Freifelder, D., Baran, N., Chud, L., Folkmanis, A., Levine, E.E.: Requirements for insertion of bacteriophage DNA into the DNA of Escherichia coli. J. Mol. Biol. 91, 401–408 (1975)
Gellert, M., Mizuuchi, K, O’Dea, M.H., Nash, H.A.: DNA gyraseGellert, M.F O’Dea, M.H., Itoh, T To miz aw a, J.; Novobiocin and courmermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc. Natl. Acad. Sci. U.S.A. 73, 4474–4478 (1976b)
Gingery, R, Echols, H.: Mutants of bacteriophage X unable to integrate into the host chromosome. Proc. Natl. Acad. Sci. U.S.A. 58, 1507–1514 (1967)
Goldberg, A.L., St. John, A.C.: Intracellular protein degradation in mammalian and bacterial cells: Part 2. Annual Review of Biochem. 45, 747–803 (1976)
Gottesman, M.E., Weisberg, R.A.: Prophage insertion and excision. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 113–138
Gottesman, M.E., Yarmolinsky, M.B.: Integration-negative mutants of bacteriophage lambda. J. Mol. Biol. 31, 487–505 (1968)
Gottesman, S., Gottesman, M.E.: Elements involved in site-specific recombination in bacteriophage lambda. J. Mol. Biol. 91, 489–499 (1975a)
Gottesman, S., Gottesman, M.: Excision of prophage A in a cell-free system. Proc. Natl. Acad. Sci. U.S.A. 72, 2188–2192 (1975b)
Guarneros, G., Echols, H.: New mutants of bacteriophage X with a specific defect in excision from the host chromosome. J. Mol. Biol. 47, 565–574 (1970)
Guarneros, G., Echols, H.: Thermal asymmetry of site-specific recombination by bacteriophage X. Virology 52, 30–38 (1973)
Henderson, D., Weil, J.: A mutant of Escherichia coli that prevents growth of phage lambda and is bypassed by lambda mutants in a nonessential region of the genome. Virology 71, 546–559 (1976)
Hendrix, R. W.: Identification of proteins coded in phage lambda. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 355–370
Honigman, A., Hu, S.-L., Chase, R., Szybalski, W.: 4S oop RNA is a leader sequence for the immunity-establishment transcription in coliphage X. Nature 262, 112–116 (1976)
Hradecna, Z., Szybalski, W.: Electron micrographic maps of deletions and substitutions in the genomes of transducing coliphages Adg and Abio. Virology 38, 473–466 (1969)
Huskey, R.J.: Deletion mutants of bacteriophage lambda. IV. High frequency int-promoted recombination. Mol. Gen. Genet. 127, 39–46 (1973)
Inman, R.B., Schnos, M.: Partial denaturation of thymine- and 5-bromouracil containing X DNA in alkali. J. Mol. Biol. 49, 93–98 (1970)
Inokuchi, H., Dove, W.F., Freifelder, D.: Physical studies of RNA involvement in bacteriophage ADNA replication and prophage excision. J. Mol. Biol. 74, 721–727 (1973)
Jovin, T.M.: Recognition mechanisms of DNA-specific enzymes. Annu. Rev. Biochem. 45, 889–920 (1976)
Kamp, D.: In vitro Untersuchung der Integrase des Bakteriophagen A. Thesis, University of Cologne, 1973
Kaiser, A.D., Masuda, T.: Evidence for a prophage excision gene in X. J. Mol. Biol. 47, 557–564 (1970)
Kaiser, A.D., Wu, R.: Structure and function of DNA cohesive ends. Cold Spring Harbor Symp. Quant. Biol. 33, 729–734 (1968)
Katzir, N., Oppenheim, A., Belfort, M., Oppenheim, A.B.: Activation of the lambda int gene by the ell and cIII gene products. Virology 74, 324–331 (1976)
Kotewicz, M., Cuhng, S., Takeda, Y., Echols, H.: Characterization of the integration protein of bacteriophage A as a site-specific DNA-binding protein. Proc. Natl. Acad. Sci. U.S.A. 74, 1511–1515 (1977)
Kourilsky, P.: Lysogenization by bacteriophage lambda and the regulation of lambda repressor synthesis. Virology 45, 853–857 (1971)
Kourilsky, P.: Lysogenization by bacteriophage lambda. II. Identification of genes involved in the multiplicity dependent processes. Biochimie 56, 1511–1516 (1974)
Kourilsky, P., Knapp, A.: Lysogenization by bacteriophage lambda. III. Multiplicity dependent phenomena occurring upon infection by lambda. Biochimie 56, 1517–1523 (1974)
Kourilsky, P., Boürguignon, M.-F., Gros, F.: Kinetics of viral transcription after induction of prophage. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 647–666.
Landy, A., Ross, W.: Viral integration and excision: structure of the lambda att sites. Science 197, 1147–1160 (1977)
Lehman, J.F.: X site-specific recombination: Local transcription and an inhibitor specified by the b2 region. Mol. Gen. Genet. 130, 333–344 (1974)
Manly, K.F., Signer, E.R., Radding, C.M.: Nonessential functions of bacteriophage X. Virology 37, 177–188 (1969)
Miller, H.I., Friedman, D.I.: Isolation of Escherichia coli mutants unable to support lambda integrative recombination. In: Plasmids, DNA Insertion Elements and Episomes. Shapiro, J., Bukhori, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory 1977, pp. 349–356
Mizuuchi, K, Nash, H.A.: Restriction assay for integrative recombination of bacteriophage X DNA in vitro: Requirement for closed circular DNA substrate. Proc. Natl. Acad. Sci. U.S.A. 73, 3524–3528 (1976)
Mizuuchi, K., Gellert, M., Nash, H.: (in preparation) (1977)
Nash, H.A.: XattB-attP, A X derivative containing both sites involved in integrative recombination. Virology 57, 207–216 (1974a)
Nash, H.A.: Purification of bacteriophage X int protein. Nature 247, 543–545 (1974b)
Nash, H.A.: Integrative recombination in bacteriophage lambda: Analysis of recombinant DNA. J. Mol. Biol. 91, 501–514 (1975a)
Nash, H.A.: Integrative recombination of bacteriophage lambda DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 72, 1072–1076 (1975b)
Nash, H.A., Merril, C.R.: DNA of biotin-transducing X bacteriophage. J. Mol. Biol. 43, 357–359 (1969)
Nash, H.A., Enquist, L., Weisberg, R.: On the role of the bacteriophage X int gene product in site specific recombination. J. Mol. Biol. 115, in press (1977 a)
Nash, H.A., Mizuuchi, K, Weisberg, R., Kikuchi, Y., Gellert, M.: Integrative recombination of bacteriophage A-The biochemical approach to DNA insertions. In: Plasmids, DNA Insertion Elements and Episomes. Shapiro, J., Bukhori, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory 1977 b, pp. 363–373
Oppenheim, A.B., Katzir, N., Oppenheim, A.: Regulation of protein synthesis in bacteriophage A: Restoration of gene expression in XN~ strains by mutations in the cro gene. Virology 79, 405–425 (1977)
Parkinson, J.S.: Deletion mutants of bacteriophage lambda. II. Genetic properties of attdefective mutants. J. Mol. Biol. 56, 385–101 (1976)
Pilacinski, W., Mosharrafa, E., Edmundson, R., Zissler, J., Fiandt, M., Szybalski, W.: Insertion sequence IS2 associated with constitutive mutants of bacteriophage lambda. Gene 2, in press (1977)
Pirrotta, V.: The A repressor and its action. Curr. Top. Microbiol. Immunol. 74, 21–54 (1976)
Ptashne, M., Backman, K, Humayun, M.Z., Jeffrey, A., Maurer, R., Meyer, B., Sauer, R.T.: Auto regulation and function of a repressor in bacteriophage lambda. Science 194, 156–161 (1976)
Ray, U., Skalka, A.: Lysogenization of Escherichia coli by bacteriophage lambda: Complementary activity of the host’s DNA polymerase I and ligase and phage replication proteins O and P. J. Virol. 18, 511–517 (1976)
Reichardt, L., Kaiser, A.D.: Control of A repressor synthesis. Proc. Natl. Acad. Sci. U.S.A. 68, 2185–2189 (1971)
Roehrdanz, R.L., Dove, W.F.: Studies of the stimulation by helper of A site-specific recombination in lytic crosses. Virology 79, 32–39 (1977a)
Roehrdanz, R.L., Dove, W.F.: A factor in the b2 region affecting site-specific recombinations in lambda. Virology 79, 40–49 (1977b)
Ryan, M.J.: Coumermycin Ax: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization. Biochemistry 15, 3769–3777 (1976)
Sadowski, P.D., Vetter, D.: Genetic recombination of bacteriophage T7 DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 73, 692–696 (1976)
Schuster, H., Beyersmann, D., Mikolajczyk, M., Schlicht, M.: Prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda. J. Virology 11, 879–885 (1973)
Sharp, P.A., Hsu, M.-T., Davidson, N.: Note on the structure of prophage A. J. Mol. Biol. 71, 499–501 (1972)
Shimada, K, Campbell, A.: Int-constitutive mutants of bacteriophage lambda. Proc. Natl. Acad. Sci. U.S.A. 71, 237–241 (1974a)
Shimada, K, Campbell, A.: Lysogenization and curing by int-constitutive mutants of phage A. Virology 60, 157–165 (1974b)
Shimada, K, Weisberg, R.A., Gottesman, M.E.: Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens J. Mol. Biol. 63, 483–503 (1972)
Shimada, K, Weisberg, R.A., Gottesman, M.E.: Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J. Mol. Biol. 80, 297–314 (1973)
Shimada, K, Weisberg, R.A., Gottesman, M.E.: Prophage lambda at unusual chromosomal locations. III. The components of the secondary attachment sites. J. Mol. Biol. 93, 415–429 (1975)
Shulman, M., Gottesman, M.: Lambda att2: a transducing phage capable of intramolecular int-xis promoted recombination. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 477–487
Shulman, M., Gottesman, M.: Attachment site mutants of bacteriophage lambda. J. Mol. Biol. 81, 461–482 (1973)
Shulman, M.J., Mizuuchi, K, Gottesman, M.M.: New att mutants of phage A. Virology 72, 13–22 (1976)
Signer, E.R.: On the control of lysogeny in phage A. Virology 40, 624–633 (1970)
Signer, E.R., Weil, J., Kimball, P.C.: Recombination in bacteriophage A. III. Studies on the nature of the prophage attachment region. J. Mol. Biol. 46, 543–563 (1969)
Skalka, A., Burgi, E., Hershey, A.D.: Segmental distribution of nucleotides in the DNA of bacteriophage lambda. J. Mol. Biol. 34, 1–16 (1968)
Smith, D.H., Davis, B.D.: Mode of action of novobiocin in Escherichia coli. J. Bacteriol. 93, 71–79 (1967)
Sobell, H.M., Tsai, C.-C., Gilbert, S.G., Jain, S.C., Sakore, T.D.: Organization of DNA in chromatin. Proc. Natl. Acad. Sci. U.S.A. 73, 3068 (1976)
Sogo, J.M., Greenstein, M., Skalka, A.: The circle mode of replication of bacteriophage lambda: The role of covalently closed templates and the formation of mixed catenated dimers. J. Mol. Biol. 103, 537–562 (1976)
Staudenbauer, W.L.: Novobiocin-A specific inhibitor of semi-conservative DNA replication in permeabilized Escherichia coli cells. J. Mol. Biol. 96, 201–205 (1975)
Syvanen, M.: In vitro genetic recombination of bacteriophage A. Proc. Natl. Acad. Sci. U.S.A. 71, 2496–2499 (1974)
Thomas, R.: Control circuits. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 211–220
Thompson, B.J., Camien, M.N., Warner, R.C.: Kinetics of branch migration in double-stranded DNA. Proc. Natl. Acad. Sci. U.S.A. 73, 2299–2303 (1976)
Tomizawa, J., Anraku, N.: Molecular mechanisms of genetic recombination in bacteriophage. IV. Absence of polynucleotide interruption in DNA of T4 and A phage particles, with special reference to heterozygosis. J. Mol. Biol. 11, 509–527 (1965)
Wang, J.C.: Interactions between twisted DNA’s and enzymes: the effects of superhelical turns. J. Mol. Biol. 87, 797–816 (1974)
Weil, J., Signer, E.R.: Recombination in bacteriophage. II. Site-specific recombination promoted by the integration system. J. Mol. Biol. 34, 273–279 (1968)
Weisberg, R.A.: Requirements for curing A lysogens. Virology 41, 195–199 (1970)
Weisberg, R.A., Gottesman, M.E.: The stability of int and xis functions. In: The Bacteriophage Lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971, pp. 489–500
Weisberg, RA., Gottesman, S., Gottesman, M.E.: Bacteriophage A: The lysogenic pathway. In: Comprehensive Virology. Frankel-Conrat, H., Wagner, R. (eds.). New York: Plenum Press 1977, Vol. Ill, pp. 197–258
Williams, J.G.K., Wulff, D.L., Nash, H.A.: A mutant of Escherichia coli deficient in a host function required for phage lambda integration and excision. In: Plasmids, DNA Insertion Elements and Episomes. Shapiro, J., Bukhari, A., Adhya, S. (eds.). New York: Cold Spring Harbor Laboratory 1977, pp. 357–361
Wulff, D.L.: Lambda CIN-1, a new mutation which enhances lysogenization by bacteriophage lambda, and the genetic structure of the lambda CY region. Genetics 82, 401–416 (1976)
Yarmolinsky, M.B.: Alternative modes of prophage insertion and excision. Adv. Biosci. 8, 31–67 (1972)
Zabin, I., Villarejo, M.R.: Protein complementation. Annu. Rev. Biochem. 44, 295–313 (1975)
Zissler, J.: Integration-negative) mutants of phage A. Virology 31, 189 (1967)
Zissler, J., Signer, E., Schaeffer, F.: The role of recombination in growth of bacteriophage lambda. I. The gamma gene. In: The Bacteriophage Lambda. Hershey, A.D. (ed.)1. New York: Cold Spring Harbor Laboratory 1971, pp. 455–475
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Nash, H.A. (1977). Integration and Excision of Bacteriophage λ. In: Arber, W., et al. Current Topics in Microbiology and Immunology. Current Topics in Microbiology and Immunology, vol 78. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66800-5_6
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