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Quantitative Analysis of Plant Hormones

  • Chapter
Hormonal Regulation of Development I

Part of the book series: Encyclopedia of Plant Physiology ((PLANT,volume 9))

Abstract

Estimates of endogenous plant hormone levels are usually obtained from analyses of solvent extracts of plant tissues. The relevance of such estimates to the true hormonal status of the plant at the time of extraction is open to question and will undoubtedly be an area of much future investigation. However, in the absence of pertinent data the scope of this article will have to be confined to questions relating to the analysis of plant extracts. The typical extract is an exceedingly complex mixture containing trace amounts of hormones at concentrations rarely greater than one part in 106. Meaningful quantitative analysis is therefore a formidable technical problem, not only in terms of the requirement for methodology possessing low limits of detection, but also because of the absolute necessity of having to distinguish the hormone of interest from the overwhelming number of other compounds present. In the following article these considerations will be discussed from a theoretical viewpoint which is then used as a basis to assess the effectiveness of procedures currently employed in the quantitative analysis of plant hormones.

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Authors
  1. D. R. Reeve
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  2. A. Crozier
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Editors and Affiliations

  1. School of Chemistry, University of Bristol, Cantock’s Close, BS8 1TS, Bristol, UK

    Jake MacMillan

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Reeve, D.R., Crozier, A. (1980). Quantitative Analysis of Plant Hormones. In: MacMillan, J. (eds) Hormonal Regulation of Development I. Encyclopedia of Plant Physiology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67704-5_4

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