Abstract
The polymorphic gene products of the human HLA region can be identified both by serological and cellular typing techniques. Although the definition of the system, its loci, and their antigenic determinants is in essence based on serological studies and analyses, it is now evident that a determinant primarily described by one technique, e.g., serology, may be detected similarly by the other, viz cellular typing (MLC, PLT, or CML). Thus, serological and cellular typing studies are equally precise with regard to specificity and must supplement each other.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Mawas C, Christen Y, Legrand L, Sasportes M, Dausset J (1974) Cellular and humoral response against determinants other than the classical HL-A specificities. Transplantation 18: 256–266
Kristensen T, Grunnet N, Kissmeyer-Nielsen F (1974) Occurrence of unexpected HL-A ( LA and FOUR) irrelevant lympholysis. Tissue Antigens 4: 378–382
Schendel DJ, Wank R, Hansen JA, Dupont B (1977) Cell-mediated lympholysis in man: identification of a common target determinant associated with HLA-B12 and Bw35. Transplant Proc 9: 1777–1780
Goulmy E, Termijtelen A, Bradley BA, van Rood J (1976) HLA restriction of non-HLA-A, -B, -C and -D cell mediated lympholysis. Tissue Antigens 8: 317–326
Biddison WE, Krangel MS, Strominger JL, Ward FE, Shearer GM, Shaw S (1980) Virus-immune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. Hum Immunol 1: 225–232
Kato S, Ivanyi P, Lacko E, Breur B, du Bois R, Eijsvoogel VP (1982) HLA-B locus (B12) antigen variants defined by CTL generated between B locus-identical (B12) responder-stimulator pairs. J Immunol 128: 949–955
Breuning MH, Lucas CJ, Breur BS, Engelsma MY, de Lange GG, Dekker AJ, Biddison WE, Iványi P (1982) Subtypes of HLA-B27 detected by cytotoxic T lymphocytes and their role in self-recognition. Hum Immunol 5: 259–268
van der Poel JJ, Mölders H, Thompson A, Ploegh HL (1983) Definition of four HLA-A2 subtypes by CML typing and biochemical analysis. Immunogenetics 17: 609–621
Goulmy E, van Leeuwen A, Blokland E, van Rood JJ, Biddison WE (1982) Major histocompatibility complex - restricted H-Y-specific antibodies and cytotoxic T lymphocytes may recognize different self determinants. J exp Med 155: 1567–1579
Tekolf AW, Biddison WE, Aster RD, Shaw S (1982) Two subgroups of HLA-Bw44 defined by cell-mediated lympholysis that differ in Bw44 expression on platelets and in patterns of genetic linkage disequilibrium. J Immunol 129: 1474–1478
Hahn AWA, Schendel DJ, Hansen PW, Ploegh HL (1984) Biochemical and cellular identification of HLA- Bw44 subtypes. Hum Immunol 11: 69–76
Gillis S, Baker PE, Ruscetti FW, Smith KA (1978) Long-term culture of human antigen specific cytotoxic T cell lines. J Exp Med 148: 1093–1101
Kristensen T, Malissen B, Johnsen HE (1982) Histocompatibility typing by polyclonal and cloned human cytotoxic T lymphocytes. In: Ferrone S, Solheim BG (eds) HLA typing: methodology and clinical aspects, vol 2. CRC Press, Florida, pp 123–150
European CML Study Group (1980) Human histocompatibility testing by T cell-mediated lympholysis: a European standard CML technique. Tissue Antigens 16: 335–367
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Hansen, P.W., Christiansen, S.E., Kissmeyer-Nielsen, F., Kristensen, T. (1984). Construction and Expansion of CTLs for HLA Typing: Definition of B44 Subtypes by Cellular Typing. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_175
Download citation
DOI: https://doi.org/10.1007/978-3-642-69770-8_175
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69772-2
Online ISBN: 978-3-642-69770-8
eBook Packages: Springer Book Archive