Summary
Osmotin is a cationic protein which accumulates (up to 12% of total cell protein) in cells adapted to grow in the medium with low water potentials. The synthesis of osmotin is developmentally regulated and is induced by abscisic acid (ABA) in cultured cells. In whole plants, both the synthesis and accumulation of osmotin is tissue specific. The highest rate of synthesis occurs in outer stem tissue and the highest level of accumulation occurs in roots. ABA induced synthesis of osmotin is transient in cells and NaCl stabilizes its synthesis and accumulation. NaCl adapted tobacco cells exhibit a stable increase in both their ability to tolerate salt and to produce osmotin in the absence of NaCl. Osmotin is localized in vacuolar inclusions but also appears to be loosely associated with the tonoplast and plasma membrane. Osmotin is also found in the culture medium of adapted cells during all stages of cell growth. The molecular weight of mature osmotin deduced from the cDNA nucleotide sequence is 23,984 daltons. Osmotin is synthesized as a preprotein 2.5 kD larger than the mature protein. Three proteins, thaumatin, TPR and MAI, exhibit a very high level (52% to 61%) of sequence homology with osmotin. Osmotin mRNA synthesis is induced by ABA. The level of osmotin mRNA increases after NaCl adaptation.
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© 1989 Springer-Verlag Berlin Heidelberg
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Singh, N.K. et al. (1989). Osmotin: A Protein Associated with Osmotic Stress Adaptation in Plant Cells. In: Cherry, J.H. (eds) Environmental Stress in Plants. NATO ASI Series, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73163-1_9
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DOI: https://doi.org/10.1007/978-3-642-73163-1_9
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