Abstract
Glutaraldehyde-tanned bovine pericardium (G-tBP) was tested for its ability to induce immunologic responses in vivo. Sections of G-tBP were implanted between the abdominal muscles in rats and guinea pigs. Control animals received Dacron implants. Lymphocytes and sera from animals were isolated at 2 and 4 weeks after implantation (four animals per group each time). Tritiated thymidine (3H-TdR) incorporation and an enzyme linked immunosorbent assay (ELISA) were used to assay T- and B-lymphocyte responses to G-tBP antigens. At the same time points implants and surrounding tissue from these animals were processed for histology. Results show that lymphocytes from animals implanted with G-tBP responded significantly (P < 0.001) to G-tBP antigens in vitro but not to Dacron. In contrast, lymphocytes from Dacron-implanted animals failed to respond to G-tBP or Dacron preparations. ELISA results show that animals implanted with G-tBP produce antibody directed against G-tBP antigens. Histology revealed a dense mononuclear cell infiltrate at the interface between G-tBP and surrounding host tissues with focal degradation of implant collagen. Dacron elicits a nonspecific lymphocytic and foreign body-type reaction. These results indicate that G-tBP can induce immunologic responses in vivo consistent with a host vs graft reaction.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Carpentier A, Lemaigre G, Robert L et al. (1969) Biological factors affecting long-term results of valvular heterografts. J Thorac Cardiovasc Surg 58: 467
O’Brien, TK, Gabbay S, Parkes AC, Knight RA, Zalesky PJ (1984) Immunological reactivity to a new glutaraldehyde tanned bovine pericardial heart valve. Trans Am Soc Artif Int Organs 30: 440–444
Ferrans JV, Boyce ME, Billingham L, Jones T, Ishihara WS, Roberts WC (1980) Calcific deposits in porcine bioprostheses: structure and pathogenesis. Am J Cardiol 46: 721–734
Ferrans JV, Spray TL, Billingham ME, Roberts WC (1978) Structural changes in glutaraldehyde-treated porcine heterografts used as substitute cardiac valves. Am J Cardiol 41: 1159–1184
Spray TL, Roberts WC (1977) Structural changes in porcine xenografts used as substitute cardiac valves. Am J Cardiol 40: 319–330
Gallo I, Nistal F, Artinano E, Fernandez D, Cayon R, Carrion M, Garcia-Martinez V (1987) The behavior of pericardial versus porcine valve xenografts in the growing sheep model. J Thorac Cardiovasc Surg 93: 281–290
Gerbode F (1970) Proceedings of the first international workshop on tissue valves 1969. Ann Surg 172 [Suppl I]: 1
Rocchini AP, Weesner KM, Heidelberger K, Keren D, Behrendt D, Rosenthal A (1981) Porcine xenograft valve failure in children: an immunologic response. Circulation 64[Suppl 11]:11–162–171
Cheung DT, Nimni ME (1982) Mechanism of crosslinking of proteins by glutaraldehyde. I. Reaction with model compounds. Connect Tissue Res 10: 187–199
Cheung DT, Nimni ME (1982) Mechanism of crosslinking of proteins by glutaraldehyde. II. Reaction with monomeric and polymeric collagen. Connect Tissue Res 10: 210–216
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Dahm, M., Lyman, W.D., Factor, S.M., Schwell, A.B., Frater, R.W.M. (1989). Immunogenicity of Glutaraldehyde-Tanned Bovine Pericardium. In: Ghosh, P.K., Unger, F. (eds) Cardiac Reconstructions. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74629-1_51
Download citation
DOI: https://doi.org/10.1007/978-3-642-74629-1_51
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-50973-8
Online ISBN: 978-3-642-74629-1
eBook Packages: Springer Book Archive