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Production of Cytokines by Human Melanoma Cells and Melanocytes

  • Conference paper
Book cover Skin Cancer: Basic Science, Clinical Research and Treatment

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 139))

Abstract

Experimental animal models have shown that various cytokines, depending on their specific properties, may support growth and metastasis of tumor cells or even lead to tumor rejection. The analysis of expression of cytokine genes by melanoma cell lines indicated that melanoma cells constitutively produce both autostimulatory and inhibitory cytokines. Using reverse transcriptase polymerase chain reaction analysis, simultaneous expression of several cytokines, including interleukin-1 beta (IL-1β), IL-6, IL-8, tumor necrosis factor-α, and granulocyte-macrophage colony-stimulating factor, by melanoma cells was found. The same cytokine transcripts were detected in melanocytes, suggesting that cells of the melanocytic lineage express a specific pattern of cytokines in vitro. All these cytokines are known to be able to stimulate effector cells of the host. Additionally, production of mRNA for IL-10, a cytokine with potential immunosuppressive properties, was detected in melanoma cells and melanocytes. These and other cytokines are likely to be involved in the immune response to cancer and at this time it is unknown what the net effects of multiple cytokines are on the outcome of the host response to tumor.

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Authors and Affiliations

  1. Department of Immunology, University Medical Center Steglitz, The Free University of Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    S. Krüger-Krasagakes & T. Diamantstein

  2. Department of Dermatology, University Medical Center Steglitz, The Free University of Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    K. Krasagakis & C. Garbe

Authors
  1. S. Krüger-Krasagakes
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  2. K. Krasagakis
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  3. C. Garbe
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  4. T. Diamantstein
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Editors and Affiliations

  1. University Department of Dermatology Medical Centre Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    Claus Garbe , Stefan Schmitz  & Constantin E. Orfanos ,  & 

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© 1995 Springer-Verlag Berlin · Heidelberg

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Krüger-Krasagakes, S., Krasagakis, K., Garbe, C., Diamantstein, T. (1995). Production of Cytokines by Human Melanoma Cells and Melanocytes. In: Garbe, C., Schmitz, S., Orfanos, C.E. (eds) Skin Cancer: Basic Science, Clinical Research and Treatment. Recent Results in Cancer Research, vol 139. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78771-3_11

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  • DOI: https://doi.org/10.1007/978-3-642-78771-3_11

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