Abstract
Capillary microinjection is efficiently used to introduce macromolecules into the nucleus or cytoplasm of living mammalian cells (Proctor 1992). Transfer can occur at well-defined stages of the cell cycle, and modifications of culture conditions are possible before, during, and after injection. The number of cells which can be injected per experiment is, however, limited. Therefore, in the past, biochemical analyses of microinjected cells has been possible, but difficult (Gautier-Rouviere et al. 1990; Lane et al. 1993).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ansorge W, Pepperkok R (1988) Performance of an automated system for capillary microinjection into living cells. J Biochem Biophys Methods 16: 283–292
Gautier-Rouviere C, Fernandez A, Lamb NJC (1990) Ras-induced c-fos expression and proliferation in living rat fibroblasts involves C-kinase activation and the serum response element pathway. EMBO J 9: 171–180
Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
Klausner RD, Donaldson JG, Lippincott-Schwartz J (1992) Brefeldin A: insights into the control of membrane traffic and organelle structure. J Cell Biol 116: 1071–1080
Kreis TE (1986) Microinjected antibodies against the cytoplasmic domain of vesicular stomatitis virus glycoprotein block its transport to the cell surface. EMBO J 5: 931–941
Lane HA, Fernandez A, Lamb NJC, Thomas G (1993) p7Osk6 function is essential for Glprogression. Nature 363:170–172
Pepperkok R, Scheel J, Horstman H, Hauri HP, Griffiths G, Kreis TE (1993a) (3-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Cell 74:71–82
Proctor GN (1992) Microinjection of DNA into mammalian cells in culture: theory and practise. Methods Mol Cell Biol 3: 209–231
Rosa P, Weiss U, Pepperkok R, Ansorge W, Niehr, C, Stelzer EHK, Huttner WB (1989) An antibody against secretogranin I (chromogranin B) is packaged into secretory granules. J Cell Biol 109: 17–34
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Pepperkok, R., Rosorius, O., Scheel, J. (1998). Cellular Microbiochemistry. In: Cid-Arregui, A., García-Carrancá, A. (eds) Microinjection and Transgenesis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80343-7_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-80343-7_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-61895-9
Online ISBN: 978-3-642-80343-7
eBook Packages: Springer Book Archive