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Growth and Cellular Characteristics of Multicell Spheroids

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Spheroids in Cancer Research

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 95))

Abstract

Experience with culture methods for multicell spheroids has demonstrated the importance of optimizing and standardizing the growth conditions for different cell types and research objectives. Spheroid growth kinetics, cellular properties, and microenvironmental characteristics are significantly influenced by spheroid cellular metabolism and the macroenvironmental conditions during the culture period. These conditions include constituents of the culture medium, frequency of replenishment of the medium, the oxygen and glucose concentrations and geometric and convection properties in the culture flask.

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Authors and Affiliations

  1. Experimental Therapeutics Division, Department of Radiation Biology and Biophysics, University of Rochester Cancer Center, 601 Elmwood Avenue, Box 704, Rochester, NY, 14642, USA

    R. M. Sutherland & R. E. Durand

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  1. R. M. Sutherland
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  2. R. E. Durand
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Editors and Affiliations

  1. Max-Plank-Institut für Systemphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, Germany

    Helmut Acker

  2. Department of Radiobiology, National Defence Research Institut, 901 82, Umea, Sweden

    Jörgen Carlsson

  3. Medical Biophysics Unit, B. C. Cancer Research Center, 601 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada

    Ralph Durand

  4. Division Experimental Therapeutics, University of Rochester, Cancer Center, 601 Elmwood Avenue, Rochester, NY, USA

    Robert M. Sutherland

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© 1984 Springer-Verlag Berlin Heidelberg

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Sutherland, R.M., Durand, R.E. (1984). Growth and Cellular Characteristics of Multicell Spheroids. In: Acker, H., Carlsson, J., Durand, R., Sutherland, R.M. (eds) Spheroids in Cancer Research. Recent Results in Cancer Research, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82340-4_2

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  • DOI: https://doi.org/10.1007/978-3-642-82340-4_2

  • Publisher Name: Springer, Berlin, Heidelberg

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