Summary
Significance, origin and nature of posttraumatic brain edema are still being debated. Recently, a “controlled cortical impact injury” (CCII) was introduced to model traumatic brain injury. Purpose of this study was to investigate the development and nature of brain edema following CCII. Traumatic brain injury was applied to the intact dura of the left hemisphere in Sprague-Dawley rats (n = 52, 250–350 g b.w.). Ketamine/xylazine-anesthesia or inhalation-anesthesia were used. A pneumatic impactor with a diameter of 5 mm contused the temporo-parietal cortex with a velocity of 7 m/s and an impact depth of 2 mm. 24 hours post injury the brains were removed. Posttraumatic hemispheric swelling and water content were determined gravimetrically, Evans blue extravasation spectrophotometri-cally, area and volume of ischemia by staining with TTC. MRI studies were performed with T1 -, T2- and diffusion-weighted sequences.
Posttraumatic swelling following CCII was 14.3 ± 3.1%. Brain water content increased to 82.5 ± 0.5% in lesioned hemisphere compared to 79.9 ± 0.2% in control hemisphere. Following TTC staining, the average ischemic tissue volume was 56.7 ± 19.2 mm3. There was a moderate uptake of Evans blue into the lesioned hemisphere. MRI studies demonstrated edema in 35.4 ± 9.5 mm3 of the lesioned hemisphere. Gd-DTPA was taken up early after trauma only. A significantly decreased ADC (apparent diffusion coefficient) indicates the cytotoxic (ischemic) component of edema in this model.
In conclusion, CCII produces significant posttraumatic brain swelling and edema which is both, of vasogenic and cytotoxic nature. Thus, the CCII models the human cortical contusion more appropriately and opens new avenues for therapeutical studies focussing on cortical contusions.
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References
Benveniste HD, Hedlund LW, Johnson GA (1992) Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy. Stroke 23: 746–754
Bullock R, Satharn P, Patterson J, et al (1990) The time course of vasogenic oedema after focal human head injury — evidence from SPECT mapping of blood brain barrier defects. Acta Neurochir (Wien) [Suppl] 51: 286–288
Dixon CE, Clifton GL, Lighthall JW, et al (1991) A controlled cortical impact model of traumatic brain injury in the rat. J Neurosci Meth 39: 253–262
Ebisu T, Naruse S, Horikawa Y, et al (1993) Discrimination between different types of white matter edema with diffusion-weighted MR imaging. J Magn Reson Imaging 3: 863–868
Hanstock CC, Faden AI, Bendall MR, et al (1994) Diffusion-weighted imaging differentiales ischemic tissue from traumatized tissue. Stroke 25: 843–848
Ito J, Marmarou A, Barzo P, et al (1996) Characterization of edema by diffusion-weighted imaging in experimental traumatic brain injury. J Neurosurg 84: 97–103
Klatzo I (1958) The relationship between edema, blood-brain barrier and tissue elements in local brain injury. J Neuropathol Exp Neurol 17:548–564
Lang DA, Hedley DM, Teasdale GM, et al (1990) Gadolinium DTPA enhanced magnetic resonance imaging in human head injury. Acta Neurochir (Wien) [Suppl] 51: 293–295
Marmarou A (1994) Traumatic brain edema: an overview. Acta Neurochir (Wien) [Suppl] 60: 421–424
Miller JD, Corales RL (1981) Brain edema as a result of head injury: fact or fallacy? In: de Vlieger M, de Lange S, Beks JWK (eds) Brain edema. Wiley, New York, pp 99–115
Todd NV, Graham DI (1990) Blood-brain barrier damage in traumatic brain contusions. Acta Neurochir (Wien) [Suppl] 51: 296–299
Unterberg A, Schneider GH, Gottschalk J, Lanksch WR (1994) Developement of traumatic brain edema in old versus young rats. Acta Neurochir (Wien) [Suppl] 60: 431–433
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© 1997 Springer-Verlag
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Unterberg, A.W., Stroop, R., Thomale, UW., Kiening, K.L., Päuser, S., Vollmann, W. (1997). Characterisation of Brain Edema Following “Controlled Cortical Impact Injury” in Rats. In: James, H.E., et al. Brain Edema X. Acta Neurochirurgica Supplements, vol 70. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6837-0_33
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DOI: https://doi.org/10.1007/978-3-7091-6837-0_33
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