Abstract
In many cases Aldolases have been proved to be useful for the synthesis of carbohydrates [1]. Here we present the optimization of reaction conditions for the application of N-acetylneuraminic acid aldolase for the synthesis of N-acetylneuraminic acid (Neu5Ac) in a continuous process which is suitable for large-scale production.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Toone EJ, Simon ES, Bednarski MD, Whitesides GM (1989) Enzyme-catalyzed synthesis of carbohydrates. Tetrahedron 45: 5365–5422
Schauer R (1982) Chemistry, metabolism, and biological functions of sialic acids. Adv Carbohydr Chem Biochem 40: 131–234
Juneja LR, Koketsu M, Nishimoto K, Kim M, Yamamoto T, Itoh T (1991) Large-scale preparation of sialic acid from chalaza and egg-yolk membrane. Carbohydr Res 214: 179–186
Augé C, Gautheron C, David S, Malleron A, Cavayé B, Bouxom B (1990) Sialyl aldolase in organic synthesis: from the trout egg acid, 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN), to branched-chain higher ketoses as possible new chirons. Tetrahedron 46: 201–214, and references cited therein
Simon ES, Bednarski MD, Whitesides GM (1988) Synthesis of CMP-Neu5Ac from N-acetyl glucosamine: generation of CTP from CMP using adenylate kinase. J Am Chem Soc 110: 7159–7163
Kragl U, Gygax D, Ghisalba O, Wandrey C (1991) Enzymatic two-step synthesis of N-acetylneuraminic acid in the enzyme membrane reactor. Angew Chem Int Ed Engl 30: 827–828, and references cited therein
Aisaka K, Igarashi A, Yamaguchi K, Uwajima T (1991) Purification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli. Biochem J 276: 541–546
Segel I (1975) Enzyme kinetics — behaviour and analysis fo rapid equilibrium and steady-state enzyme systems. John Wiley & Sons. New York
Deijl CM, Vliegenthart JFG (1983) Configuration of substrate and products of N-acetylneuraminate pyruvate-lyase from Clostridium perfringens. Biochem Biophys Res Commun 111: 668–674
Kragl U, unpublished results
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Tokyo
About this paper
Cite this paper
Kragl, U., Appel, P., Gygax, D., Ghisalba, O., Wandrey, C. (1992). Aldolases for Use in Carbohydrate Synthesis: Enzymatic Reaction Engineering as a Tool for Process Optimization. In: Furusaki, S., Endo, I., Matsuno, R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_20
Download citation
DOI: https://doi.org/10.1007/978-4-431-68180-9_20
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68182-3
Online ISBN: 978-4-431-68180-9
eBook Packages: Springer Book Archive