Abstract
From information presented in previous chapters it will be clear to readers that plant hormones are, as a rule, present at very low levels in most plant tissues. Whilst relatively high levels of some hormones are found in immature seeds of certain species (e.g., GAs in developing pea seeds (10)) even these levels are low when compared with the levels of most plant secondary metabolites. Thus while many alkaloids, terpenoids and phenolics may be present at levels of mgs. per gm. dry weight of plant material, plant hormones are usually present at several hundred to several thousand fold lower levels. It is not suprising therefore that knowlege of the chemical identity of plant hormones has been limited by the techniques available for their isolation in a pure state and by the sensitivity of the spectroscopic techniques required to elucidate their chemical structure.
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References
Albone, K.S., Gaskin, P., MacMillan, J., Sponsel, V.M. (1984) Identification and localisation of gibberellins in maturing seeds of the cucurbit Sechium edule, and a comparison between this cucurbit and the legume Phaseolus coccineus. Planta 162, 560–565.
Badenoch-Jones, J., Summons, R.E., Entsch, B., Rolfe, B.G., Parker, C.W., Letham, D.S. (1982) Mass spectrometric identification of indole compounds produced by Rhizobium strains. Biomed. Mass Spectrometry 9, 430–436.
Biddington, N.L. and Thomas, T.H. (1973) A modified A maranthus betacyanin bioassay for the rapid determination of cytokinins in plant tissues. Planta 111, 183–186.
Binks, R., MacMillan, J., Pryce, R.J. (1969) Plant hormones VIII. Combined gas chromatography-mass spectrometry of the methyl esters of gibberellins A1 to A24 and their trimethylsilyl ethers. Phytochemistry 8, 271–284.
Bowman, W.R., Linforth, R.S.T., Rossall, S., Taylor, I.B. (1984) Accumulation of an ABA analogue in the wilty tomato mutant, flacca. Biochemical Genetics 22, 369–378.
Crozier, A., Loferski, J., Zaerr, J., Morris, R.O. (1980) Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures. Planta 150, 366–370.
Durley, R.C., Kannangara, T., Simpson, G.M. (1982) Leaf analysis for abscisic, phaseic and 3-indolylacetic acids by high performance liquid chromatography. J.Chromatography 236, 181–188.
Ehmann, A. (1974) Identification of 2-0-(indole-3-acetyl)-D- glucopyranose, 4-0-(indole- 3-acetyl)-D-glucopyranose and 6-0- (indole-3-acetyl)-D-glucopyranose from kernels of Zea mays by gas liquid chromatography-mass spectrometry. Carbohydrate Research 34, 99–114.
Engelhart, H. (1978) High Perfomance Liquid Chromatography. Springer-Verlag, Berlin.
Frydman, V.M., Gaskin, P., MacMillam, J. (1974) Qualitative and quantitative analysis of gibberellins throughout seed maturation in Pisum sativum cv. Progress No.9. Planta 118, 123–132.
Gray, R.T., Mallaby, R., Ryback, G., Williams, V.P. (1974) Mass spectra of methyl abscisate and isotopically labelled analogues. J. Chem. Soc Perkin Trans. 2, 919–924.
Horgan, R., Kramers, M.R. (1979) High-performance liquid chromatography of cytokinins. J.Chromatography 173, 263–270.
Ingram, T.J., Reid, J.B., Murfet, I.C., Gaskin, P., Willis, C.L., MacMillan, J. (1984) Internode length in Pisum. The Le gene controls the 3-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160, 455–463.
Koshioka, M., Harada, J., Takeno, K., Noma, M., Sassa, T., Ogiyama, K., Taylor, J.S., Rood, S.B., Legge, R.L., Pharis, R.P. (1983) Reverse phase C18 high-performance liquid chromatography of acidic and conjugated gibberellins. J. Chromatography 256, 101–115.
Letham, D.S., Shannon, J.C., MacDonald, I.R.C. (1964) The structure of zeatin, a (kinetin like) factor inducing cell division. Proc. Chem. Soc. London 230–231.
MacLeod, J.K., Summons, R.E., Letham, D.S. (1976) Mass spectrometry of cytokinin metabolites. Per(trimethylsilyl) and permethyl derivatives of glucosides of zeatin and 6- benzylaminopurine. J.Org.Chem. 41, 3959–3967.
MacMillan, J., Pryce, R.J., Eglinton, G., McCormick, A. (1967) Identification of gibberellins in crude plant extracts by combined gas chromatography-mass spectrometry. Tetrahedron Lett. 2241–2243.
Magnus, V., Bandurski, R.S., Schulze, A. (1980) Synthesis of 4,5,6,7 and 2,4,5,6,7 deuterium labelled indole-3-acetic acid for use in mass spectrometric assays. Plant Physiol. 66, 775–781.
Millard, B.J. (1979) Quantitative mass spectrometry. Heyden, London.
Miller, C. O. (1963) Kinetin and kinetin-like compounds. In: Modern methods of plant analysis. Linskins, H.F. and Tracey, M. V. eds. Vol. VI, pp 194–202. Springer: Berlin- Heidelberg- New York.
Morris, R.O. (1977) Mass spectrometric identification of cytokinins. Glucosyl zeatin and glucosyl ribosylzeatin from Vinca rosea crown gall. Plant Pysiol. 59, 1029–1033.
Murakami, Y. (1968) The microdrop method, a new rice seedling test for gibberellins and its use for testing extracts of rice and morning glory. Bot. Mag. 79, 33–43.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Neill, S.J., Horgan, R., Heald, J.K. (1983) Determination of the levels of abscisic acid- glucose ester in plants. Planta 257, 371–375.
Neill, S.J., Horgan, R., Lee, T.S., Walton, D.C. (1981) 3- methyl-5(4 -oxo-2,6,6 - trimethylcyclohex-2 -en-1 -yl)-2,4 pentadienoic acid from Cercospora rosicola. FEBS Letters 128, 30–32.
Phinney, B.O. (1956) Growth response of single-gene mutants of maize to gibberellic acid. Proc. Natl. Acad. Sci. USA. 42, 185–186.
Reeve, D.R., Crozier, A. (1978) The analysis of gibberellins by high performance liquid chromatography, in Isolation of Plant Growth Substances, SEB Seminar Series, Vol. 4 ed. J.R. Hillman, Cambridge University Press, pp. 41–77.
Rose, M.E., Johnson, R.A.W. (1982) Mass spectrometry for chemists and biochemists. Cambridge University Press.
Saunders, P.F. (1978) The identification and quantitative analysis of abscisic acid in plant extracts, in Isolation of Plant Growth Substances, SEB Seminar Series, Vol.4 ed. J.R.Hillman, pp. 115–134.
Scott, I.M., Horgan, R. (1984) Mass spectrometric quantification of cytokinin nucleotides and glycosides in tobacco crown gall tissue. Planta 161, 345–354.
Scott, I.M., Horgan, R., McGaw, B.A. (1980) Zeatin-9-glucoside a major endogenous cytokinin of Vinca rosea L. crown gall tissue. Planta 149, 472–475.
Udea, M., Bandurski, R.S. (1974) Structure of indole-3-acetic acid myoinositol esters and pentamethyl-myoinositols. Phytochemistry 13, 243–253.
Van Staden, J., Drewes, S.E. (1974) Identification of cell division inducing compounds from coconut milk. Physiol. Plant. 32, 347–352.
Yokota, T., Murofushi, N., Takahashi, N. (1980) Extraction, purification and identification, in Encyclopedia of Plant Physiology, Vol. 9, Molecular aspects of plant hormones, ed. J.MacMillan, Springer-Verlag, Berlin, pp. 113–201.
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Horgan, R. (1987). Instrumental Methods of Plant Hormone Analysis. In: Davies, P.J. (eds) Plant Hormones and their Role in Plant Growth and Development. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3585-3_12
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DOI: https://doi.org/10.1007/978-94-009-3585-3_12
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