Summary
An increasing number of woody plant species can be axenically propagated using plant tissue culture methods. These techniques produce high quality healthy plants but transplant problems often arise due to their being susceptible to different environmental stresses. Mycorrhizae can play a vital role in plant nutrition and survival and their introduction during the production of axenically propagated plants could contribute to the success of this technique. Using a simple method for axenic establishment and direct observation of VA mycorrhizal cultures, in vitro mycorrhizal synthesis of a woody plant species has been obtained for the first time using axenically propagated plants of Prunus avium L. (wild cherry) and the VA fungus Gigaspora margarita.
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References
Allen M F, Moore Jr T S, Christensen M and Stanton N 1979 Growth of vesicular-arbuscular mycorrhizal and nonmycorrhizal Bouteloua gracilis in a defined medium. Mycologia 71, 666–669.
Becker W N and Hall I R 1976 Gigaspora margarita, a new species in the Endogonaceae. Mycotaxon 4, 155–160.
Boullard B 1968 Les Mycorhizes. Masson and Co. Paris, 135 p.
Covey R P, Koch B L and Larsen H J 1981 Influence of vesicular-arbuscular mycorrhizae on the growth of apple and corn in low-phosphorus soil. Phytopathology 71, 712 – 715.
Crush J R and Hay M J M 1981 A technique for growing mycorrhizal clover in solution culture. N. Z. J. Agric. Res. 24, 371 – 372.
Green N E, Graham S O and Schenck N C 1976 The influence of pH on the germination of vesicular-arbuscular mycorrhizal spores. Mycologia 68, 929 – 934.
Harley J L 1969 The Biology of Mycorrhiza. 2nd edition Leonard Hill, London, 334 p.
Hepper CM 1981 Techniques for studying the infection of plants by vesicular-arbuscular mycorrhizal fungi under axenic conditions. New Phytol. 88, 641 – 647.
Kleinschmidt G D and Gerdemann J W 1972 Stunting of citrus seedlings in fumigated soils in relation to the absence of endomycorrhizas. Phytopathology 62, 1447 – 1453.
Kormanik P P, Bryan W C and Schultz R 1976 Endomycorrhizae: their importance in nursery production of hardwood seedlings. Proc. SE Area Nurserymen’s Conf. Eastern Session, Charleston, USA pp 18 – 21.
Lambert D H, Stouffer R F and Cole Jr H 1979 Stunting of peach seedlings following soil fumigation. J. An. Soc. Hortic. Sci. 104, 433–435.
Menge J A, Johnson E L V and Piatt R G 1978 Mycorrhizal dependency of several citrus cultivars under three nutrient regimes New Phytol. 81, 553 – 559.
Morandi D, Gianinazzi S and Gianinazzi-Pearson V 1979 Intérêt de l’endomycorrhization dans la reprise et la croissance du Framboisier issu de multiplication végétative in vitro. Ann. Amélior. Plantes 29, 623–630.
Mosse B 1959 The regular germination of resting spores and some observations on the growth requirements of an Endogone sp. Trans. Brit. Mycol. Soc. 46, 273 – 286.
Mosse B, Stribtley D P and Le Tacon F 1981 Ecology of mycorrhizae and mycorrhizal fungi. In Advances in Microbiol. Ecology. Ed. Alexander, Plenum Press, N. Y. and London pp 137 – 210.
Navatel J C 1980 L’utilisation des cultures in vitro pour la multiplication de quelques espèces légumières et fruitières. C. R. Acad. Agric. France 66, 681 – 696.
Navatel JC 1982 Multiplication végétative in vitro: intérêt et problèmes liés peut-être au défaut de mycorhization du matériel obtenu. Les Colloques de l’I.N.R.A. 13, 339 – 343.
Németh G 1981 Milieux de multiplication végétative in vitro Table Ronde sur la Multiplication in vitro d’Espèces Ligneuses 6–7 June 1978. C.R.A.-Gembloux, B. 39 – 42.
Ollivier B, Diem H G, Pinta M and Dommergues Y R 1982 Effetdel’endomycorhizationsurla concentration en phosphore et en zinc des parties aeriennes de Vigna unguiculata cultivé dans un sol Dek. Les Colloques de l’I.N.R.A. 13, 155 – 161.
Phillips J M and Hayman D S 1970 Improved procedures for clearing and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158 – 161.
Plenchette C, Furlan V and Fortin J A 1981 Growth stimulation of apple trees in unsterilized soil under field conditions with VA mycorrhiza inoculation. Can. J. Bot. 59, 2003–2008.
Pons F, Gianinazzi-Pearson V and Gianinazzi S 1982 Synthèse in vitro des endomycorhizes ericoîdes et VA: complément à la micropropagation. Les Colloques de l’I.N.R.A. 13, 345 – 349.
Quoirin M, Lepoivre Ph and Boxus Ph 1977 Un premier bilan de 10 années de recherches sur les cultures de méristèmes et la multiplication in vitro de fruitiers ligneux. C. R. Rech. 1976–1977. Rapp. Synth. Stn. Cuit, fruitières maraîchères Gembloux, B. 93 – 117.
Walkey D G 1972 Production ofappleplantlets from axillary-budmeristems. Can. J. Plant Sci. 52, 1085 – 1087.
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© 1983 Martinus Nijhoff/Dr W. Junk Publishers, The Hague
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Pons, F., Gianinazzi-Pearson, V., Gianinazzi, S., Navatel, J.C. (1983). Studies of VA mycorrhizae in vitro: mycorrhizal synthesis of axenically propagated wild cherry (Prunus avium L.) plants. In: Atkinson, D., Bhat, K.K.S., Coutts, M.P., Mason, P.A., Read, D.J. (eds) Tree Root Systems and Their Mycorrhizas. Developments in Plant and Soil Sciences, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6833-2_22
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DOI: https://doi.org/10.1007/978-94-009-6833-2_22
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