Skip to main content
SpringerLink
Log in

Epithelial Transport of Human Secretory Immunoglobulins

  • Chapter
The Mucosal Immune System

Part of the book series: Current Topics in Veterinary Medicine and Animal Science ((CTVM,volume 12))

  • 50 Accesses

Abstract

According to the proposed transport model, the J (‘joining’) chain and epithelial SC (‘secretory component’) represent ‘the lock and key’ in the selective external translocation of dimeric IgA and pentameric IgM through serous-type secretory epithelial cells. Incorporation of J chains into these two immunoglobulin isotypes during their production in gland-associated immunocytes apparently induces a configurational fit (binding site) allowing them to combine with SC in the plasma membrane of the epithelial cell. This complexing on the basolateral surface of the cell seems to stimulate pinocytosis (adsorptive endocytosis); the completed secretory IgA and secretory IgM molecules with bound SC are then transported in cytoplasmic vesicles to the gland lumen along with an excess of free SC. The following observations that support the proposed transport model will be discussed in some detail: 1. Immunoglobulin-binding properties of SC; 2. Characteristics of the immunoglobulin products of gland-associated immunocytes; 3. Localisation of SC and immunoglobulins in normal and neoplastic secretory epithelium.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bourne, F.J.:, 1974. Structural features of pig IgA. Immunol. Commun. 3, 157

    CAS  Google Scholar 

  • Brandtzaeg, P., 1968. Glandular secretion of immunoglobulins. Acta pathol. microbial. scand. 74, 624.

    Article  Google Scholar 

  • Brandtzaeg, P., 1971a. Human secretory immunoglobulins. II. Salivary secretions from individuals with selectively excessive or defective synthesis of serum immunoglobulins. Clin. exp. Immunol. 8, 69.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1971b. Human secretory immunoglobulins. III. Immunochemical and physicochemical studies of secretory IgA and free secretory piece. Acta path. microbial. scand. Section B 79, 165.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1971c. Human secretory immunoglobulins. VII. Concentrations of parotid IgA and other secretory proteins in relation to the rate of flow and duration of secretory stimulus. Arch. oral Biol. 16, 1295.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1973a. Structure, synthesis and external transfer of mucosal immunoglobulins. Ann. Immunol. ( Inst. Pasteur ). 124c, 417.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1973b. Two types of IgA immunocytes in man. Nature New Biol. (Land.) 243 142.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1974a. Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol fixation technique. Immunology. 26, 1101.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1974b. Mucosal and glandular distribution of immunoglobulin components. Differential localisation of free and bound SC in secretory epithelial cells. J. Immunol. 112, 1553.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1974c. Characteristics of SC-Ig complexes formed in Vitro, In: The Immunoglobulin A System (ed. by J. Mestecky and A.R. Lawton) (Adv. exp. Med. Biol. 45 ), pp. 87–97, Plenum Press, New York.

    Google Scholar 

  • Brandtzaeg, P., 1974d. Presence of J chain in human immunocytes containing various immunoglobulin classes. Nature (Land.) 252, 418.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1975a. Human secretory immunoglobulin M. An immunochemical and immunohistochemical study. Immunology 29, 559.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1975b. Blocking effect of J chain and J-chain antibody on the binding of secretory component to human IgA and IgM. Scand. J. Immunol. 4, 837.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1975c. Immunochemical studies on free and bound J chain of human IgA and IgM. Scand. J. Immunol. 4, 439.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1975d. Human secretory component. IV. Aggregation and fragmentation of free secretory component. Immunochemistry 12, 877.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1976a. Complex formation between secretory component and human immunoglobulins related to their content of J chain. Scand. J. Immunol. 5, 411.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1976b. Studies on J chain and binding site for secretory component in circulating human B cells. II. The cytoplasm. Clin. exp. Immunol. 25, 59.

    CAS  Google Scholar 

  • Brandtzaeg, P., 1977a. Human secretory component. VI. Immunoglobulinbinding properties. Immunochemistry. 14, 178.

    Article  Google Scholar 

  • Brandtzaeg, P., 1977b. Immunohistochemical studies on various aspects of glandular immunoglobulin transport in man. Histochem. J. 9, 553.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., 1978. Polymeric IgA is complexed with secretory component (SC) on the surface of human intestinal epithelial cells. Scand. J. Immunol. 8, 39.

    Article  PubMed  CAS  Google Scholar 

  • Brandtzaeg, P., 1981. Development of systemic and local gut immune responses. In: International Conference on Infant Nutrition and Diarrhoeal Disease (ed. by N. Iyngkaran) in press, Kuala Lumpur.

    Google Scholar 

  • Brandtzaeg, P. and Baklien, K., 1976a. Inconclusive immunohistochemistry of human IgE in mucosal pathology. Lancet. 1, 1297.

    Article  PubMed  CAS  Google Scholar 

  • Brandtzaeg, P. and Baklien, K., 1976b. Immunohistochemical studies of the formation and epithelial transport of immunoglobulins in normal and diseased human intestinal mucosa. Scand. a. Gastroenterol. 11, Suppl. 36, pp. 1–45.

    CAS  Google Scholar 

  • Brandtzaeg, P., Fjellanger, I. and Gjeruldsen, S.T., 1968. Immunoglobulin M: local synthesis and selective secretion in patients with immunoglobulin A deficiency. Science ( Wash. DC ). 160, 789.

    Article  CAS  Google Scholar 

  • Brandtzaeg, P., Fjellanger, I. and Gjeruldsen, S.T., 1970. Human secretory immunoglobulins. I. Salivary secretions from individuals with normal or low levels of serum immunoglobulins. Scand. J. Haematol. Suppl. 12, pp. 1–83.

    Google Scholar 

  • Brandtzaeg, P., Gjeruldsen, S.T., Korsrud, F., Baklien, K., Berdal, P. and Ek, J., 1979. The human secretory immune system shows striking heterogeneity with regard to involvement of J-chain-positive IgD immunocytes. J. Immunol. 122 503.

    PubMed  CAS  Google Scholar 

  • Brown, W.R., Isobe, Y. and Nakane, P.K., 1976. Studies on translocation of immunoglobulins across intestinal epithelium. II. Immuncelectron-microscopic localisation of immunoglobulins and secretory component in human intestinal mucosa. Gastroenterology. 71, 985.

    CAS  Google Scholar 

  • Bull, D.M., Bienenstock, J. and Tomasi, T.B., 1971. Studies on human intestinal immunoglobulin A. Gastroenterology. 60, 370.

    PubMed  CAS  Google Scholar 

  • Chen, S.-T., 1971. Cellular sites of immunoglobulins. II. The relative proportions of mucosal cells containing IgG, IgA and IgM, and light polypeptide chains of kappa and lamda immunoglobulin in human appendices. Acta Pathol. Jap. 21, 67.

    CAS  Google Scholar 

  • Crago, S.S., Kuhhavy, R., Prince, S.J. and Mestecky, J., 1978. Secretory component on epithelial cells is a surface receptor for polymeric immunoglobulins. J. exp. Med. 147, 1832.

    Article  PubMed  CAS  Google Scholar 

  • Ejeckam, G.C., Huang, S.N., McCaughey, W.T.E. and Gold, P., 1979. Immunohistopathologic study on carcinoembryonic antigen ( CEA)-like material and immunoglobulin A in gastric malignancies. Cancer. 44, 1606.

    Article  PubMed  CAS  Google Scholar 

  • Eskeland, T. and Brandtzaeg, P., 1974. Does J chain mediate the combination of 19S IgM and dimeric IgA with the secretory component rather than being necessary for their polymerisation? Immunochemistry. 11, 161.

    CAS  Google Scholar 

  • Grubb, A.O., 1978. Quantitation of J chain in human biological fluids by a simple immunochemical procedure. Acta med. scand. 204, 453.

    CAS  Google Scholar 

  • Haneburg, B., 1974a. Human faecal agglutinins to rabbit erythrocytes Scand. J. Immunol. 3, 71.

    Google Scholar 

  • Haneburg, B., 1974b. Immunoglobulins in faeces from infants fed human or bovine milk. Scand. J. Immunol. 3, 191.

    Article  Google Scholar 

  • Haneburg, B. and Aarskog, D., 1975. Human faecal immunoglobuline in healthy infants and children, and in some with diseases affecting the intestinal tract or the immune system. Clin. exp. Immunol. 22, 210.

    Google Scholar 

  • Hanson, L. Å. and Brandtzaeg, P., 1980. The mucosal defense system. In: Immunological disorders in Infants and Children, Ed. 2 (ed. By R.E. Stiehm and V.A. Fulginiti ) pp. 137–164. W.B. Saunders Co., London.

    Google Scholar 

  • Heremans, J.F. and Crabbé, P.A., 1967. Immunohistochemical studies on exocrine IgA. In: Gammaglobulins (ed. by J. Killander ) pp. 129–139. Almgvist and Wiksell, Uppsala.

    Google Scholar 

  • Huang, S.W., Fogh, J. and Hong, R., 1976. Synthesis of secretory component by colon cancer cells. Scand. J. Immunol. 5, 263.

    Article  PubMed  CAS  Google Scholar 

  • Jerry, L.M., Kunkel, H.G. and Adams, L., 1972. Stabilisation of dis-sociable IgA2 proteins by secretory component. J. Immunol. 109, 275.

    PubMed  CAS  Google Scholar 

  • Jos, J., Labbe, F., Geny, B. and Griscelli, C., 1979. Immunoelectronmicroscopic localisation of immunoglobulin A and secretory component in jejunal mucosa from children with coeliac disease. Scand. J. Immunol. 9, 441.

    Article  PubMed  CAS  Google Scholar 

  • Kemler, R., Mossman, H., Strohmaier, K., Kickhöfen, B. and Hammer, D.K., 1975. In vitro studies on the selective binding of IgG from different species to tissue sections of the bovine mammary gland. Eur. J. Immunol. 5, 603.

    Article  PubMed  CAS  Google Scholar 

  • Knight, K.L., Vetter, M.L. and Malek, T.R., 1975. Distribution of covalently bound and non-covalently bound secretory component on subclasses of rabbit secretory IgA. J. Immunol. 115, 595.

    PubMed  CAS  Google Scholar 

  • Korsrud, F.R. and Brandtzaeg, P., 1980. Quantitative immunohistochemistry of immunoglobulin-and J-chain-producing cells in human parotid and submandibular glands. Immunology. 39, 129.

    PubMed  CAS  Google Scholar 

  • Koshland, M.E., 1975. Structure and function of the J chain. Adv. Immunol. 20, 41.

    Article  PubMed  CAS  Google Scholar 

  • Kraehenbuhl, J.P. and KÜhn, L., 1978. Transport of immunoglobulins across epithelia. In: Transport of Macromolecules in Cellular Systems (ed. by S.C. Silverstein) pp. 213–228. Life Science Research Report 11.

    Google Scholar 

  • Kraehenbuhl, J.P., Racine, L. and Galardy, R.E., 1975. Localisation of secretory IgA, secretory component, and a heavy chain in the mammary gland of lactating rabbits by immunoelectron microscopy. Ann. N.Y. Acad. Sci. 254, 190.

    Article  PubMed  CAS  Google Scholar 

  • Kühn, L. and Kraehenbuhl, J.P., 1979a. Interaction of rabbit secretory component with rabbit IgA dimer, J. biol. Chem. 254, 11066.

    Google Scholar 

  • Kühn, L.C. and Kraehenbuhl, J.P., 1979b. Role of secretory component, a secreted glycoprotein, in the specific uptake of IgA dimer by epithelial cells. J. biol. Chem. 254, 11072.

    Google Scholar 

  • Lamm, M.E., 1976. Cellular aspects of immunoglobulin A. Adv. Immunol. 22, 223.

    Article  PubMed  CAS  Google Scholar 

  • Lindh, E., 1974. Increased resistance of immunoglobulin A dimers to proteolytic degradation after binding of secretory component. J. Immunol. 114, 284.

    Google Scholar 

  • Lindh, E. and Björk, I., 1976a. Binding of secretory component to human immunoglobulin M. Europ. J. Biochem. 62, 271.

    Article  CAS  Google Scholar 

  • Lindh, E. and Björk, I., 1976b. Binding of secretory component to dimmers of immunoglobulin A in vitro. Mechanism of the covalent bond formation. Eur. J. Etochem. 62, 263.

    CAS  Google Scholar 

  • Lindh, E. and Björk, I., 1977. Relative rates of the non-covalent and covalent binding of secretory component to an IgA dimer. Acta path. mirobiol. scand, Sect. C. 85, 449.

    Google Scholar 

  • Mach, J.P., 1970. In vitro combination of human and bovine free secretory component with IgA of various species. Nature (Lond.) 228, 1278.

    Article  CAS  Google Scholar 

  • Maeda, S., 1977. Studies of immunoglobulins in gastric juice and gastric mucosa. IV. Studies on secretory component in gastric mucosa. Sappora med. J. 46, 409.

    CAS  Google Scholar 

  • Mestecky, J., Kraus, F.W. and Voight, S.A., 1970. Proportion of human colostral immunoglobulin A molecules containing the secretory determinant. Immunology. 18, 237.

    PubMed  CAS  Google Scholar 

  • Mosmann, T.R., Gravel, Y., Williamson, A.R. and Baumal, R., 1978. Modification and fate of J chain in myeloma cells in the presence and absence of immunoglobulin secretion. Eur. J. Immunol. 8, 94.

    Article  PubMed  CAS  Google Scholar 

  • Munster, P.J.J. van, 1972. De secretoire component. Een immunochemisch onderzoek. Isolatie, eigenschappen, uitscheiding in secreta en localisatie in jejunumbiopten. (The secretory component. An immunochemical investigation. Isolation, properties, excretion in secretions and localisation in jejunal biopsy samples.) Thesis, University of Nijmegen.

    Google Scholar 

  • Murkofsky, N.A. and Lamm, M.E., 1979. Effect of a disulfide-exchange enzyme on the assembly of human secretory immunoglobulin A from immunoglobulin A and free secretory component. J. biol. Chem. 254, 12181.

    PubMed  CAS  Google Scholar 

  • Nagura, H., Brandtzaeg, P., Nakane, P.K. and Brown, N.R., 1979a. Ultrastructuräl localisation of J chain in human intestinal mucosa. J. Immunol. 123, 1044.

    CAS  Google Scholar 

  • Nagura, H., Nakane, P.K. and Brown, W.R., 1979b. Translocation of dimeric IgA through neoplastic colon cells in vitro. J. Immunol. 123, 2359.

    PubMed  CAS  Google Scholar 

  • Nakajima, S., Gillespie, D.N. and Gleich, G.J., 1975. Differences between IgA and IgE as secretory proteins. Clin. exp. Immunol, 21, 306.

    PubMed  CAS  Google Scholar 

  • Newcomb, R.W. and Ishizaka, K., 1970. Physiocochemical and antigenic studies on human E in respiratory fluid. J. Immunol. 105, 85.

    CAS  Google Scholar 

  • Newmark, P., 1979. Pathways to secretion. Nature (London). 281, 629.

    Article  CAS  Google Scholar 

  • O’Daly, J.A., Craig, S.W. and Cebra, J.J., 1971. Localisation of b markers, a,-chain and SC of sIgA in epithelial cells lining Lieberkuhn crypts. J. Immunol. 166, 286.

    Google Scholar 

  • Poger, M.E., Hirsch, B.R. and Lamm, M.E., 1976. Synthesis of secretory component by colonic neoplasms. Am. J. Path. 82, 327.

    PubMed  CAS  Google Scholar 

  • Poger, M.E. and Lamm, M.E., 1974. Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. E. exp. Med. 139, 629.

    Article  CAS  Google Scholar 

  • Radl, J., Klein, F., van der Berg, P., de Bruyn, A.M. and Hijmans, W., 1971. Binding of secretory piece to polymeric IgA and IgM paraproteins in vitro. Immunology. 20,843.

    PubMed  CAS  Google Scholar 

  • Raff, M.C., 1976. Cell-surface immunology. Scient. Am. 234, 30.

    Article  CAS  Google Scholar 

  • Richman, L.K. and Brown, W.R., 1977. Immunochemistry characterization of IgM in intestinal fluids. J. Immunol. 119, 1515.

    PubMed  CAS  Google Scholar 

  • Rodewald, R., 1976. Intestinal transport of peroxidase-conjugated IgG fragments in the neonatal rat. In: Maternofoetal Transmission of Immunoglobulins. (ed. by W.A. Hemmings ) p. 137–153, Cambridge University Press, London.

    Google Scholar 

  • Rognum, T., Brandtzaeg, P., Ørjasaeter, H., Elgjo, K. and Hognestad, J.,1980. Immunohistochemical study of secretory component, secretory IgA and carcinoembryonic antigen in large bowel carcinomas. Pathol. Res. Pract. (in press).

    Google Scholar 

  • Savilahti, E., 1973. IgA deficiency in children. Immunoglobulin-containing cells in the intestinal mucosa, immunoglobulins in secretions and serum IgA levels. Clin. exp. Immunol. 13, 395.

    PubMed  CAS  Google Scholar 

  • Schiff, J.M., Dorrington, K.J. and Underdown, B.J., 1980. Secretory component and immunoglobulin transport. Abstract No. 7.5.29 presented at 4th International Congress of Immunology, Paris.

    Google Scholar 

  • Singer, S.J. and Nicolson, G.L., 1972. The fluid mosaic model of the structure of cell membranes. Science (Wash DC) 175, 720.

    Article  CAS  Google Scholar 

  • Socken, D.J. and Underdown, B.J., 1978. Comparison of human, bovine and rabbit secretory component-immunoglobulin interactions. Immunohistochemistry. 15, 499.

    Article  CAS  Google Scholar 

  • South, M.A., Cooper, M.D., Wollheim, F.A., Hong, R. and Good, R.A., 1966. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J. exp. Med. 123, 615.

    Article  PubMed  CAS  Google Scholar 

  • Tomasi, T.B. and Czerwinski, D.S., 1976. Naturally occurring polymers of IgA lacking J chain. Scand. J. Immunol. 5, 647.

    Article  PubMed  CAS  Google Scholar 

  • Tomasi, T.B., Tan, E.M., Solomon, A. and Prendergast, R.A., 1965. Characteristics of an immune system common to certain external secretions. J. exp. Med. 121 101.

    Article  PubMed  CAS  Google Scholar 

  • Weicker, J. and Underdown, B.J., 1975. A study on the association of human secretory component with IgA and IgM proteins. J. Immunol. 114, 1337.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Histochemical Laboratory, Institute of Pathology, The National Hospital, Rikshospitalet, Oslo, Norway

    P. Brandtzaeg

Authors
  1. P. Brandtzaeg
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Animal Husbandry, Langford House, Langford, Bristol, UK

    F. J. Bourne

Rights and permissions

Reprints and permissions

Copyright information

© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg

About this chapter

Cite this chapter

Brandtzaeg, P. (1981). Epithelial Transport of Human Secretory Immunoglobulins. In: Bourne, F.J. (eds) The Mucosal Immune System. Current Topics in Veterinary Medicine and Animal Science, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8331-1_5

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-8331-1_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-8333-5

  • Online ISBN: 978-94-009-8331-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation