Abstract
We have focussed considerable attention on simplifying production using the baculovirus expression system in batch culture. In this work we show that we can achieve yields, of our model β-Galactosidase protein, using an MOI as low as 10-4 PFU/cell and a TOI of 3 x 105 cells/ml, comparable to yields achieved when infecting at much higher cell densities (TOI of 3-4 x 106 cells/ml, MOI of 2-5).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baker, K., Zheng, Y-Z., Reid, S. and Greenfield, P.F. (1993). In: Animal Cell Technology: Basic and Applied Aspects, Kaminogawa, S., Ametani, A. and Hachimura, S. (eds), Kluwer Academic Publishers, Dordrecht, 521–528.
Cavegn, C. and Bernard, A. 1992. In: Baculovirus and Recombinant Protein Production Processes, Vlak, J.M., Schlaeger, E-J and Bernard, A.R. (eds) Editions Roche, Basel, Switzerland, 262–273.
de Gooijer, C.D., van Lier, F.L.J., van den End, E.J., Vlake, J.M. and Tramper, J. 1989. Appl. Microbiol. Biotechnol, 30, 497–501.
Guillaume, J.M., Couteault, N., Hunvitz, D.R. and Crespo, A. 1992. In: Baculovirus and Recombinant Protein Production Processes, Vlak, J.M., Schlaeger, E-J and Bernard, A.R. (eds), Editions Roche, Basel, Switzerland, 285–296.
Kompier, R., Tramper, J. and Vlak, J.M. 1988. Biotech. Letters, 10, 849–854.
Kool, M., Voncken, J.W., van Lier, F.L.J., Tramper, J. and Vlak, J.M. 1991.Virology, 183, 739–746.
Miller, J.H., 1972. In: Experiments in Molecular Genetics. Cold Spring Harbour Laboratory, Cold Spring Harbour, N.Y. pp. 352–355.
Nielsen, L.K., Smyth, G.K. and Greenfield, P.F. 1992.Cytotechnology 8, 231–236.
Power J.F.Reid, S., Radford, K.M., Greenfield, P.F. and Nielsen, L.K. 1994. Biotech. Bioeng. (in press).
Radford, K.M., Reid, S. and Greenfield, P.F. 1993. The Sixth Annual Meeting of the Japanese Association for Animal Cell Technology, November 9–12, Nagoya, Japan.
Summers, M.D. and Smith, G.E. 1987. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas A & M University, Texas Agricultural Expt. Station, Bulletin No. 1555.
van Lier, F.L.J., van den End, E.J., de Gooijer, C.D., Vlak, J.M. and Tramperk, J. (1990). Appl. Microbiol. Biotechnol., 33, 43–47.
Weiss, S.A., Godwin, G.P., Whitford, W.G., Gorfien, S.F. and Dougherty, E.M. 1992. Proceedings, 10th Australian Biotechnology Conference, Editor: I.G. Prince, Melbourne, Australia, 67–71.
Wickham, T.J., Davis, T.., Granados, R.R., Hammer, D.A., Shuler, M.L. and Wood, H.A. 1991. Biotech Letters, 13, 483–488.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Reid, S. et al. (1995). The Use of Very Low Multiplicities of Infection with the Baculovirus Expression Vector System. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_12
Download citation
DOI: https://doi.org/10.1007/978-94-011-0437-1_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
Online ISBN: 978-94-011-0437-1
eBook Packages: Springer Book Archive