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Regulation of Nitrogen Assimilation in the Cyanobacterium Synechococcus

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Nitrogen Fixation

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 48))

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Abstract

Synechococcus is a unicellular cyanobacterium able to assimilate nitrate, nitrite or ammonium. Cellular elements involved in the assimilation of these compounds are subjected to repression by ammonium. Ammonium also promotes an immediate inhibition of the nitrate transport system. We have recently identified a gene (ntcA) whose product appears to be required for the expression of genes subjected to repression by ammonium. Mutants that constitutively express elements of the nitrate assimilation system have also been reported, suggesting that negative regulatory elements might also be involved in the control of nitrogen assimilation in Synechococcus.

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References

  1. Andriesse, X., Van Arkel, G., and Weisbeek, P. (1990) ‘Cloning of the nitrate reductase gene of Synechococcus PCC 7942’, in Nitrate Assimilation: Molecular and Genetic Aspects (Third International Symposium), Bombannes ( France ), Abstracts p. 98.

    Google Scholar 

  2. Boussiba, S., Dilling, W., and Gibson, J. (1984) ‘Methylammonium transport in Anacystis nidulans R2’, J. Bacteriol. 160, 204–210.

    PubMed  CAS  Google Scholar 

  3. Boussiba, S. and Gibson, J. (1987) ‘Regulation of methylammonium/ ammonium transport in the unicellular cyanobacterium Synechococcus R2 (PCC 7942)’, FEMS Microbiol. Lett. 43, 289–293.

    Article  CAS  Google Scholar 

  4. Candau, P. (1979) ‘Purificación y propiedades de la ferredoxina-nitrato reductasa de la cianobacteria Anacystis nidulans’, Ph. D. Thesis, Universidad de Sevilla.

    Google Scholar 

  5. Candau, P., Manzano, C, and Losada, M. (1976) ‘Bioconversion of light energy into chemical energy through reduction with water of nitrate to ammonia’, Nature 262, 715–717.

    Article  CAS  Google Scholar 

  6. Florencio, F. J. and Ramos, J. L. (1985) ‘Purification and characterization of glutamine synthetase from the unicellular cyanobacterium Anacystis nidulans’, Biochim. Biophys. Acta 838, 39–48.

    Article  CAS  Google Scholar 

  7. Flores, E., Guerrero, M. G., and Losada, M. (1980) ‘Short-term ammonium inhibition of nitrate utilization by Anacystis nidulans and other cyanobacteria’, Arch. Microbiol. 128, 137–144.

    Article  CAS  Google Scholar 

  8. Flores, E., Guerrero, M. G., and Losada, M. (1983) ‘Photosynthetic nature of nitrate uptake and reduction in the cyanobacterium Anacystis nidulans’, Biochim. Biophys. Acta 722, 408–416.

    Article  CAS  Google Scholar 

  9. Flores, E., Herrero, A., and Guerrero, M. G. (1987) ‘Nitrite uptake and its regulation in the cyanobacterium Anacystis nidulans’, Biochim. Biophys. Acta 896, 103–108.

    Article  CAS  Google Scholar 

  10. Flores, E., Ramos, J. L., Herrero, A., and Guerrero, M. G. (1983) ‘Nitrate assimilation by cyanobacteria’, in G. C. Papageorgiou and L. Packer (eds.), Photosynthetic Prokaryotes: Cell Differentiation and Function, Elsevier Science Publishing Co., New York, pp. 363–387.

    Google Scholar 

  11. Flores, E., Romero, J. M., Guerrero, M. G., and Losada, M. (1983) ‘Regulatory interaction of photosynthetic nitrate utilization and carbon dioxide fixation in the cyanobacterium Anacystis nidulans’, Biochim. Biophys. Acta 725, 529–532.

    Article  CAS  Google Scholar 

  12. Harrison, M. A., Keen, J. N., Findlay, J. B. C, and Allen, J. F. (1990) ‘Modification of a glnB-like gene product by photosynthetic electron transport in the cyanobacterium Synechococcus 6301’, FEBS Lett. 264, 25–28.

    Article  PubMed  CAS  Google Scholar 

  13. Herrero, A., Flores, E., and Guerrero, M. G. (1981) ‘Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719’, J. Bacteriol. 145, 175–180.

    PubMed  CAS  Google Scholar 

  14. Herrero, A., Flores, E., and Guerrero, M. G. (1984) ‘Regulation of the nitrate reductase level in Anacystis nidulans: activity decay under nitrogen stress’, Arch. Biochem. Biophys. 234, 454–459.

    Article  PubMed  CAS  Google Scholar 

  15. Herrero, A. and Guerrero, M.G. (1986) ‘Regulation of nitrite reductase in the cyanobacterium Anacystis nidulans’, J. Gen. Microbiol. 132, 2463–2468.

    CAS  Google Scholar 

  16. Kuhlemeier, C, Logtenberg, T., Stoorvogel, W., Van Heugten, H. A. A., Borrias, W. E., and Van Arkel, G. A. (1984) ‘Cloning of two nitrate reductase genes from the cyanobacterium Anacystis nidulans’, J. Bacteriol. 159, 36–41.

    PubMed  CAS  Google Scholar 

  17. Kuhlemeier, C. J., Teeuwsen, V. J. P., Janssen, M. J. T., and Van Arkel, G. A. (1984) ‘Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle gene library’, Gene 31, 109–116.

    Article  PubMed  CAS  Google Scholar 

  18. Lara, C, Romero, J. M., and Guerrero, M. G. (1987) ‘Regulated nitrate transport in the cyanobacterium Anacystis nidulans’, J. Bacteriol. 169, 4376–4378.

    PubMed  CAS  Google Scholar 

  19. Madueño, F., Borrias, W. E., Van Arkel, G. A., and Guerrero, M. G. (1988) ‘Isolation and characterization of Anacystis nidulans R2 mutants affected in nitrate assimilation: establishment of two new mutant types’, Mol. Gen. Genet. 213, 223–228.

    Article  Google Scholar 

  20. Madueño, F., Flores, E., and Guerrero, M. G. (1987) ‘Competition between nitrate and nitrite uptake in the cyanobacterium Anacystis nidulans’, Biochim. Biophys. Acta 896, 109–112.

    Article  Google Scholar 

  21. Madueño, F., Vega-Palas, M. A., Flores, E., and Herrero, A. (1988) ‘A cytoplasmic-membrane protein repressible by ammonium in Synechococcus R2: altered expression in nitrate-assimilation mutants’, FEBS Lett. 239, 289–291.

    Article  Google Scholar 

  22. Manzano, C. (1977) ‘La reducción fotosintética del nitrato en el alga verde-azulada Anacystis nidulans’, Ph. Thesis, Universidad de Sevilla.

    Google Scholar 

  23. Manzano, C, Candau, P., Gómez-Moreno, C., Relimpio, A., and Losada, M. (1976) ‘Ferredoxin-dependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans’, Mol. Cell. Biochem. 10: 161–169.

    Article  PubMed  CAS  Google Scholar 

  24. Ornata, T. (1990) ‘Cloning, nucleotide sequence and mutagenesis of the nrtA gene encoding the 45-kD protein involved in nitrate transport in the cyanobacterium Synechococcus PCC 7942’, in Nitrate Assimilation: Molecular and Genetic Aspects (Third International Symposium), Bombannes ( France ), Abstracts pp. 54–57.

    Google Scholar 

  25. Ornata, T., Ohmori, M., Arai, N., and Ogawa, T. (1989) ‘Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport’, Proc. Natl. Acad. Sci. USA 86, 6612–6616.

    Article  Google Scholar 

  26. Romero, J. M., Flores, E., and Guerrero, M. G. (1985) ‘Inhibition of nitrate utilization by amino acids in intact Anacystis nidulans cells’, Arch. Microbiol. 142, 1–5.

    Article  PubMed  CAS  Google Scholar 

  27. Romero, J. M., Lara, C., and Guerrero, M. G. (1985) ‘Dependence of nitrate utilization upon active CO2 fixation in Anacystis nidulans: a regulatory aspect of the interaction between photosynthetic carbon and nitrogen metabolism’, Arch. Biochem. Biophys. 237, 396–401.

    Article  PubMed  CAS  Google Scholar 

  28. Serrano, A. and Losada, M. (1988) ‘Action spectra for nitrate and nitrite assimilation in blue-green algae’, Plant Physiol. 86, 1116–1119.

    Article  PubMed  CAS  Google Scholar 

  29. Sivak, M. N., Lara, C, Romero, J. M., Rodriguez, R., and Guerrero, M. G. (1989) ‘Relationship between a 47-kDa cytoplasmic membrane polypeptide and nitrate transport in Anacystis nidulans’, Biochem. Biophys. Res. Commun. 159, 257–262.

    Article  Google Scholar 

  30. Stevens, S. E. and Van Baalen, C. (1973) ‘Characteristics of nitrate reduction in a mutant of the blue-green alba Agmenellum quadruplicatum’, Plant Physiol. 51, 350–356.

    Article  PubMed  CAS  Google Scholar 

  31. Tischner, R. and Schmidt, A. (1984) ‘Light mediated regulation of nitrate assimilation in Synechococcus leopoliensis’, Arch. Microbiol. 137, 151–154.

    Article  CAS  Google Scholar 

  32. Vega-Palas, M. A., Madueño, F., Herrero, A., and Flores, E. (1990) ‘Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942’, J. Bacteriol. 172, 643–647.

    PubMed  CAS  Google Scholar 

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© 1991 Springer Science+Business Media Dordrecht

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Herrero, A., Vega-Palas, M.A., Flores, E. (1991). Regulation of Nitrogen Assimilation in the Cyanobacterium Synechococcus . In: Polsinelli, M., Materassi, R., Vincenzini, M. (eds) Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3486-6_85

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  • DOI: https://doi.org/10.1007/978-94-011-3486-6_85

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5541-3

  • Online ISBN: 978-94-011-3486-6

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