Abstract
Ascochyta blight is currently the most severe fungal disease of field pea (Pisum sativum L.) in Australia. Lathryus sativus (grasspea) has been found to contain the highly sought-after resistance genes for aschochyta blight, though attempts have failed at introducing useful traits from this species into field pea through conventional crossing (Pang, Brown, 1993). Protoplast isolation from field pea has been described for commercial cultivars (Lehminger-Mertens, Jacobsen, 1989; Puonti-Kaerlas, Eriksson, 1988) but never for a wild genotype. A protocol for obtaining protoplasts from L. sativus has not been published previously. This paper describes protocols for establishing a culture system for field pea protoplasts from a wild genotype, the establishment of shoot, callus and suspension cultures of L. sativus, and subsequent protoplast isolation. Protocols for electrofusion are also described.
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© 1999 Springer Science+Business Media Dordrecht
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McCutchan, J.S., Larkin, P.J., Stoutjesdijk, P.A., Pang, E.C.K., Taylor, P.W.J. (1999). Electrofusion of Field Pea and Lathryus Protoplasts for Hybrid Development. In: Altman, A., Ziv, M., Izhar, S. (eds) Plant Biotechnology and In Vitro Biology in the 21st Century. Current Plant Science and Biotechnology in Agriculture, vol 36. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4661-6_41
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DOI: https://doi.org/10.1007/978-94-011-4661-6_41
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