Abstract
In recent years, there has been increasing interest in developing a complete, highvolume, DNA analysis system using microfabrication techniques. Such analysis systems require components for injection, pumping, mixing, reaction, separation and detection. Over the past decade, many researchers have demonstrated that micromachined fluidic devices are capable of performing many of the required functions of chemical analysis. In addition, coupling the individual analysis steps onto a single microfabricated device significantly simplifies the process, minimizes human intervention, reduces the risk of contamination, and could lead to the realization of hand-held ‘Lab-on-a-chip’ devices. We are presently developing a fluidic handling, reaction and separation system for integrated DNA analysis. The current generation of our system contains an injection system based on selective hydrophobic patterning, air driven/thermocapillary fluid pump, a temperature controlled reaction chamber and a high-resolution electrophoresis and detection system. Since all the components are fabricated on the same wafer and use a similar channel design, the entire device could potentially function as one unit. We have successfully used these devices to perform a variety of DNA analysis techniques including constant temperature amplification using Strand Displacement Amplification (SDA) and gel-electrophoresis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Manz et al., Planar chip technology for miniaturization and integration of separation techniques into monitoring systems: Capillary Electrophoresis on a chip, J. Chromat., 593 (1992) 253–258.
D. J. Harrison, A. Manz, Z. Fan, H. Ludi, and H. M. Widmer, Capillary electrophoresis and sample injection systems integrated on a planar glass chip, Anal. Chem., 64 (1992) 1926–1932.
Kurt Seiler, D. Jed Harrison, and A. Manz, Planar glass chips for capillary electrophoresis: repetitive sample injection, quantitation and separation efficiency, 65 (1993) 1481–1488.
A. T. Woolly and R. A. Mathies, Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips, Proc. Natl. Acad. Sci. USA., 91 (1994) 11348–11352.
C. S. Effenhouser, A. Paulus, A. Manz, and H. M. Widmer, High-speed separation of antisense oligonucleotides on a micromachined capillary electrophoresis device, Analytical Chemistry, 66 (1994) 2949–2953.
M. A. Northrup, M. T. Ching, R. M. White, R. T. Watson, DNA amplification with a microfabricated reaction chamber, 7th International Conference on Solid State Sensors and Actuators, (1993) 924–926.
M. A. Burns et al., Microfabricated structures for integrated DNA analysis, Proc. Natl. Acad. Sci. USA, 93 (1996) 5556–5561.
L.C. Watters et al., Microchip Device for Cell Lysis, Multiplex PCR amplification, and electrophoretic sizing, Anal. Chem., 70, (1998) 158–162.
S. C. Jacobsen and J.M. Ramsey, Integrated microdevice for DNA restriction fragment analysis, Anal. Chem., 68 (1996) 720–723.
K. Handique; B. P. Gogoi, D. T. Burke, C. H. Mastrangelo, and M. A. Burns, Microfluidic flow control using selective hydrophobic patterning, Proceedings of SPIE Conference on Micromachined Devices and Components, 3224 (1997) 185–195.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Brahmasandra, S.N. et al. (1998). A Microfabricated Fluidic Reaction and Separation System for Integrated DNA Analysis. In: Harrison, D.J., van den Berg, A. (eds) Micro Total Analysis Systems ’98. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5286-0_63
Download citation
DOI: https://doi.org/10.1007/978-94-011-5286-0_63
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6225-1
Online ISBN: 978-94-011-5286-0
eBook Packages: Springer Book Archive