Abstract
3D cell culturing has become attractive in biology and tissue engineering laboratories as it mimics the natural environment for the cells to grow, differentiate and interact in all directions. To study cells and cellular interactions within 3D, cell culture requires a non-invasive, non-toxic, and high resolution imaging technique. The existing imaging techniques face challenges to image cells in 3D macro-scale environment because of the sample size, photo-bleaching or resolution requirements. Optical projection tomography (OPT) is a non-invasive 3D imaging technique for samples in the range of 1-10 mm. It works in both emission and transmission modes for fluorescence and bright-field imaging, respectively. Here, we demonstrate the use of OPT for imaging of cells and cellular materials in 3D gellan gum hydrogel. Fluorescence projection images showed alive and dead human lung fibroblast cells encapsulated in hydrogel. The mineralized extracellular matrix secreted by the human adipose stem cells in the hydrogel was evenly distributed throughout the sample and analyzable in 3D volume.
This is a preview of subscription content, log in via an institution to check access.
Preview
Unable to display preview. Download preview PDF.
References
1. Susan Breslin, Lorraine et al. (2013) Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today 18:240-249
2. Kyllonen L, Haimi S, Mannerstrom B et al. (2013) Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro. Stem Cell Research and Therapy 15:4-17
3. J. Koivisto et al. (2017) Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering. Biomedical Materials (in press)
4. Louise E. Smith, Rod Smallwood, Sheila Macneil et al. (2010) A comparison of imaging methodologies for 3d tissue engineering. Microsc. Res. Tech 73: 1123-1133
5. Kalle Lehto, Iilmari Tamminen, Soile Nymark et al. (2014) 3D-imaging of rat retina using contrast enhanced X-ray microtomography.In:Abstract of ISIE ARVO imaging conference, Orlando, Florida, 4-8 May 2014
6. James Sharpe, U. Ahlgren, P. Perry et al. (2002) Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 296: 541–545
7. E. Figueiras, Ana M. Soto, Danilo Jesus et al. (2014) Optical projection tomography as a tool for 3D imaging of hydrogels. Biomed. Opt. Express 5: 3443-3449
8. James Sharpe et al. (2004) Optical projection tomography. Annu. Rev. Biomed. Eng 6:209 –228
9. Ana M. Soto, Janne T. Koivisto, Jenny E. Parraga et al. (2016) Optical projection tomography technique for image texture and mass transport studies in hydrogels based on gellan gum. American Society of Chemistry 32 : 5173–5182
10. Castillo Diaz LA, Elsawy M, Saiani A et al. (2016) Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel. J Tissue Eng. 7:1-15
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Belay, B. et al. (2018). Optical Projection Tomography Imaging of Single Cells in 3D Gellan Gum Hydrogel. In: Eskola, H., Väisänen, O., Viik, J., Hyttinen, J. (eds) EMBEC & NBC 2017. EMBEC NBC 2017 2017. IFMBE Proceedings, vol 65. Springer, Singapore. https://doi.org/10.1007/978-981-10-5122-7_249
Download citation
DOI: https://doi.org/10.1007/978-981-10-5122-7_249
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-5121-0
Online ISBN: 978-981-10-5122-7
eBook Packages: EngineeringEngineering (R0)