Abstract
Gellan gum is a hydrogel with potential for soft tissue engineering but a quick and thorough method is needed for screening of different possible compositions for more extensive studies. Here optical projection tomography in bright field mode was used to image nearly transparent hydrogels to record their optical texture in 3D. The gained Haralick’s textural features were then analyzed with multiple discriminant analysis and combined with data from mechanical testing and neuronal cell culturing. We show the usefulness of optical texture analysis in screening of hydrogel compositions when aiming for tissue engineering applications.
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References
Annabi N, Tamayol A, Uquillas JA et al. (2014) 25th anniversary article: Rational design and applications of hydrogels in regenerative medicine. Adv Mater 26:85-124 DOI 10.1002/adma.201303233.
Koivisto J T, Joki T, Parraga J et al. (2017) Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering. Biomed Mater DOI 10.1088/1748-605X/aa62b0.
Bacelar A H, Silva-Correia J, Oliveira JM et al. (2016) Recent progress on gellan gum hydrogels provided by functionalization strategies. J Mater Chem B 4:6164-6174 DOI 10.1039/C6TB01488G.
Soto A M, Koivisto JT, Parraga JE et al. (2016) Optical projection tomography technique for image texture and mass transport studies in hydrogels based on gellan gum. Langmuir 32:5173-5182 DOI 10.1021/acs.langmuir.6b00554.
Sharpe J, Ahlgren U, Perry P et al. (2002) Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 296:541-545 DOI 10.1126/science.1068206.
Haralick R M, Shanmuga K and Dinstein I (1973) Textural features for image classification. IEEE T Syst Man Cyb SMC3: DOI 10.1109/TSMC.1973.4309314.
Duda R O, Hart PE and Stork DG (2001) Pattern Classification. Wiley, New York (NY).
Figueiras E, Soto AM, Jesus D et al. (2014) Optical projection tomography as a tool for 3D imaging of hydrogels. Biomedical Optics Express 5:3443-3449 DOI 10.1364/BOE.5.003443.
Gupta S and Markey MK (2005) Correspondence in texture features between two mammographic views. Med Phys 32:1598-1606 DOI 10.1118/1.1915013.
Lappalainen R S, Salomäki M, Ylä-Outinen L et al. (2010) Similarly derived and cultured hESC lines show variation in their developmental potential towards neuronal cells in long-term culture. Regener Med 5:749-762 DOI 10.2217/rme.10.58.
Schneider C A, Rasband WS and Eliceiri KW (2012) NIH image to ImageJ: 25 years of image analysis. Nat Meth 9:671-675.
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Koivisto, J.T. et al. (2018). Texture-property relations of bioamine crosslinked gellan gum hydrogels. In: Eskola, H., Väisänen, O., Viik, J., Hyttinen, J. (eds) EMBEC & NBC 2017. EMBEC NBC 2017 2017. IFMBE Proceedings, vol 65. Springer, Singapore. https://doi.org/10.1007/978-981-10-5122-7_48
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DOI: https://doi.org/10.1007/978-981-10-5122-7_48
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