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Introduction to Spectrophotometric Techniques

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Essentials of Pharmaceutical Analysis

Abstract

Spectrometric techniques are used to measure the interaction of different frequency components of electromagnetic radiations (EMR) with that of matter. After interaction with matter, these radiations are absorbed by the matter. It is not possible that we look at the matter instead, we observe the interaction of light with different degrees of freedom of matter and/or substance. This chapter describes the basics of spectrophotometry and various types of spectrophotometric techniques. Spectrophotometry is a key method that is frequently used for identification and quantification of raw materials and pharmaceutical products.

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Further Reading

  • Beckett A, Stenlake J (1997) Practical pharmaceutical chemistry, part II, vol 1. CBS Publications and Distributors, New Delhi, pp 275–300

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  • Gauglitz G, Dakin JP (2017) Spectroscopic analysis. In: John PD, Robert GWB (eds) Handbook of optoelectronics, vol 2. CRC Press, Boca Raton, pp 569–600

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  • Gauglitz G, Moore DS, Vo-Dinh T (2014) Handbook of spectroscopy. Wiley, Hoboken

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  • LibreTexts™. Introduction to spectroscopy. Accessed 2 Sept 2019

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  • Pavia DL, Lampman GM, Kriz GS, Vyvyan JA (2014) Introduction to spectroscopy. Cengage Learning, Boston

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  • Sudha PC (2012) Pharmaceutical analysis. Pearson Education India, Chennai

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  • Waters Corporation. Introduction to spectroscopy. Accessed 10 Aug 2019

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  • Watson DG (2015) Pharmaceutical analysis E-book: a textbook for pharmacy students and pharmaceutical chemists. Elsevier Health Sciences, Amsterdam

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Government College University, Faisalabad, Pakistan

    Muhammad Sajid Hamid Akash

  2. Department of Pharmacy, University of Agriculture, Faisalabad, Pakistan

    Kanwal Rehman

Authors
  1. Muhammad Sajid Hamid Akash
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  2. Kanwal Rehman
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Akash, M.S.H., Rehman, K. (2020). Introduction to Spectrophotometric Techniques. In: Essentials of Pharmaceutical Analysis. Springer, Singapore. https://doi.org/10.1007/978-981-15-1547-7_2

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