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CRISPR/Cas-Based Insect Resistance in Crops

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CRISPR Crops

Abstract

Insects are responsible for considerable crop losses worldwide through their direct damage and the transmission of various diseases as well. Recently, novel techniques would replace the most frequently used chemical insecticides and help facilitate the sustainability in crop production in near future. Different strategies to overcome crop resistance against insect, especially Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated system (CRISPR/Cas) based genome editing and gene drives are becoming available for insecticide resistance management. Recent advances and applications of CRISPR/Cas9 both in plants and insects offer promising mechanism of deterrence to insect pests through improving resistance of Bt, knockout, or insertion of new genes; in depth understanding of plant response against insect pests provides routes to optimize plant defenses against insects. In addition, directed evolution may play an important role to combat insect resistance against Bt crops. Although, various genome editing techniques have been developed, however; CRISPR-based approaches for insect management in crops are growing rapidly so far. Therefore, recently CRISPR-mediated gene drives are being established as potential insect management approaches in agriculture.

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Authors and Affiliations

  1. Entomology Lab, Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan

    Muhammad Kashif Zahoor, Muhammad Zulhussnain & Kanwal Ranian

  2. Center for Advanced Studies in Agriculture and Food Security (CASAFS), University of Agriculture, Faisalabad, Pakistan

    Aftab Ahmad

  3. Department of Microbiology, Government College University Faisalabad, Faisalabad, Pakistan

    Muhammad Asif Zahoor

  4. Department of Biochemistry, Government College Women University Faisalabad, Faisalabad, Pakistan

    Humara Naz Majeed

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  1. Muhammad Kashif Zahoor
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  2. Aftab Ahmad
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  3. Muhammad Asif Zahoor
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  4. Humara Naz Majeed
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  5. Muhammad Zulhussnain
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Correspondence to Muhammad Kashif Zahoor .

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  1. Center for Advanced Studies in Agriculture and Food Security (CASAFS), University of Agriculture, Faisalabad, Pakistan

    Aftab Ahmad

  2. Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan

    Sultan Habibullah Khan

  3. Institute of Plant Breeding and Biotechnology, MNS University of Agriculture, Multan, Pakistan

    Zulqurnain Khan

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Zahoor, M.K., Ahmad, A., Zahoor, M.A., Majeed, H.N., Zulhussnain, M., Ranian, K. (2021). CRISPR/Cas-Based Insect Resistance in Crops. In: Ahmad, A., Khan, S.H., Khan, Z. (eds) CRISPR Crops. Springer, Singapore. https://doi.org/10.1007/978-981-15-7142-8_4

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