Abstract
Optimization of culture conditions has been proposed for high-frequency shoots regeneration by in vitro propagation of cotyledonary node segments (CN) attained from 7-day-old aseptic seedlings in a highly valuable medicinal plant—Withania somnifera L. Micropropagation was accomplished from CN explants as bared to diverse range of cytokinins viz; 6-benzyl adenine (BA), kinetin (Kn), or 2-isopentenyl adenine (2iP) individually or in combined arrangement with various auxins namely indole 3-acetic acid (IAA), indole-3-butyric acid, or α-naphthalene acetic acid (NAA) in Murashige and Skoog medium. Bud break was dependent on cytokinin supply and the maximum rate (95%) of regeneration with highest number of shoots (13.0 ± 0.00) and shoot length (2.00 ± 0.36 cm) was evident on MS medium composed of BA (2.5 μM), after 4 weeks of incubation. However, the synergistic combination of cytokinin, BA (2.5 μM) along with auxin NAA (0.5 μM) encouraged maximum number (29.3 ± 0.23) of shoots and shoot length (5.62 ± 0.17 cm) with 90% regeneration frequency after 8 weeks of culture. After 4 weeks, the greatest number 12.6 ± 0.10 of roots with root length of 3.58 ± 0.16 cm/shoot with 95% regeneration frequency were attained on medium fortified with NAA (0.5 μM). The results highlight the potential conservation approaches for constant furnish of plant materials for both local and escalating markets as well as safety evaluation on sustainable usage of the species for medicinal purposes.
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Abbreviations
- 2-iP:
-
2-Isopentenyl adenine
- BA:
-
6-Benzyladenine
- CN:
-
Cotyledonary node
- IBA:
-
Indole-3-butyric acid
- Kin:
-
6-Furfurylaminopurine
- MS:
-
Murashige and Skoog’s medium
- NAA:
-
α-Naphthalene acetic acid
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Acknowledgments
The financial assistance in the form of UGC-Post Doctoral Fellowship for Women Candidates (UGC-PDFWM 2017-18) to Dr. Nigar Fatima funded by University Grants Commission (UGC), Government of India, New Delhi, is deeply acknowledged. Moreover, the award of UGC-BSR Faculty Fellowship (2017) by The University Grants Commission, New Delhi to Prof. Mohammad Anis is greatly acknowledged.
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Fatima, N., Anis, M. (2021). Regulation of In Vitro Morphogenesis by Modulation of Culture Conditions in Withania somnifera L. Using Cotyledonary Node Explants. In: Siddique, I. (eds) Propagation and Genetic Manipulation of Plants. Springer, Singapore. https://doi.org/10.1007/978-981-15-7736-9_9
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